Comparison Of Collagen Content In Velvet Antler At Different Growth Stages And Optimization Of Extraction Conditions
Keywords
Deer Antler, Collagen, Extraction, Optimization
Abstract
Using sika deer antler as raw material, the collagen content and collagen extraction effect in velvet antler at different growth stages were studied and the appropriate extraction conditions were determined. The pepsin method was used to extract collagen, and the Box-Behnken central combination experimental design was used to optimize the collagen extraction conditions. The results show that the collagen content and yield in Ergongzhi are higher than those of Trichosanthes trichophylla and Trichosanthes trichosanthes, which are 65.8mg/g and 4.53% respectively. The suitable process conditions for collagen extraction are extraction time of 52h, enzyme dosage of 5%, and liquid-to-material ratio. 23:1 (mL/g), under these conditions, the yield of velvet antler collagen is 5.92%
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Original research was done by Wang Shanshan, Zhao Yuhong, Jin Xiuming
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