Extraction And Characteristics Of Collagen From The Skirt Of Chinese Soft-Shell Turtle
Keywords
Chinese Soft-Shell Turtle, Hem, Collagen, Extraction, Purification
Abstract
Collagen is an important protein in living organisms and the main protein component of connective tissue. Compared with terrestrial animals (such as pigs, cattle, etc.), aquatic animal collagen has excellent physical and chemical properties and physiologically active functions, which has attracted widespread attention. Chinese soft-shell turtle is a traditional precious aquatic product in my country. Its skirt is rich in collagen and has extremely high medicinal and nutritional value. Pepsin enzymatic hydrolysis method was used to extract Chinese soft-shell turtle skirt collagen under acidic conditions. The effects of enzyme addition, substrate concentration, and extraction time on the extraction effect were studied. The optimal conditions for preparing skirt collagen were determined to be: enzyme addition amount. 4mg/ml, substrate concentration 4%, extraction time 14h. The crudely extracted collagen was purified through salting out, dialysis and other purification steps, and then analyzed the biochemical characteristics of the collagen through SDS-page electrophoresis, thermal denaturation temperature measurement, ultraviolet and Fourier transform infrared scanning and other technical methods. The results showed that the extracted skirt collagen The protein has high purity, contains two ¦Á chains and one ¦Â chain, and is a typical type I collagen. Research shows that Chinese soft-shell turtle skirt collagen can be used as an effective substitute for pig skin and cowhide collagen, which provides a scientific theoretical basis for further comprehensive development and utilization of turtle skirt collagen peptides in the future.
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Original research was done by Lu Jianfeng, Wan Quan, Yin Zhangmin, Lin Lin, Weng Shibing, Ye Yingwang, Jiang Shaotong
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