Effect Of Cross-Linking Method On The Biological Properties Of Grass Carp Skin Collagen Sponge
Keywords
Grass Carp, Fish Skin, Collagen, Cross-Linked
Abstract
In order to explore the effects of different cross-linking methods on the properties of grass carp skin collagen sponge materials, the experiment used grass carp skin as raw material to extract and purify collagen and prepare collagen sponge materials. On this basis, the collagen sponge was treated with UV cross-linking, thermal cross-linking, glutaraldehyde cross-linking and EDC/NHS cross-linking methods respectively, and the cross-linking degree, thermal denaturation temperature, tensile strength and in vitro enzyme resistance of the material were measured. Degradation performance, the cross-linking effects of different methods were compared. The results showed that the extracted grass carp skin collagen was typical type I collagen; after cross-linking treatment by different methods, the cross-linking degree of the sponge material was in the order of glutaraldehyde (72.0%) > edc/nhs (32.5%) > thermal cross-linking cross-linking (29.9%) > UV cross-linking (15.6%); compared with the control collagen material, after glutaraldehyde treatment, the thermal denaturation peak temperature (67.4¡ãC), maximum tensile strength (125.6kpa) and in vitro resistance of the collagen material The enzymatic degradation properties are significantly improved (p<0.05); after edc/nhs treatment, the thermal denaturation enthalpy of the collagen material is significantly improved (6.86j/g), while the tensile strength (98.6kpa) and in vitro anti-enzymatic degradation properties of the material A modest increase was also obtained (p<0.05). UV cross-linking and thermal cross-linking have limited effects on improving the properties of grass carp skin collagen materials, and may lead to partial denaturation of collagen molecules. The analysis results of infrared spectrum show that glutaraldehyde treatment can lead to the formation of new covalent cross-links in the triple helix molecules of grass carp skin collagen, thereby improving the material properties, while the EDC/NHS treatment mainly leads to the formation of new hydrogen bonding cross-links between collagen molecules. The combination can improve the stability of collagen materials. Studies have shown that glutaraldehyde and EDC/NHS cross-linking can effectively improve the properties of collagen sponge materials, while thermal cross-linking and UV cross-linking have very limited effects on improving material properties. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Wang Haibo, Liang Yanping, Li Yunyan, Wang Min, Fang Cheng, Wang Haiying
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