Study On The Extraction, Modification And Properties Of Rabbit Skin Type I Collagen
Keywords
Rabbit Skin, Collagen, Acid Soluble, Enzyme Soluble, Separation
Abstract
Collagen was extracted from rabbit skin using a combination of acid dissolution and enzyme dissolution. It was qualitatively analyzed by SDS-PAGE, and its physical and chemical properties were characterized by FT-IR and TG/DTG. The results showed that the extracted collagen was Type I collagen maintains a complete triple helix structure; during the heat treatment process, there is obvious weight loss three times before 100¤@¡æ, between 200~450¤@¡æ and after 450¤@¡æ. After glutaraldehyde cross-linking modification, The water content of the collagen was (78.7¡À1.3)%, and the enzyme degradation rate dropped from (88.65¡À0.11)% to (6.03¡À0.38)% (p<0.01). The cell growth inhibition method was used to determine the effect of modified collagen on mice. Cytotoxicity of embryonic fibroblasts (MEF-WT). The results show that the modified collagen has no obvious toxicity to MEF-WT cells, and the toxicity evaluation is level 0 or 1. The collagen can be used in tissue engineering. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Feng Wenpo, Qi Yuanming, Tang Keyong
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