Preliminary Study On The Use Of Collagen Chitosan To Construct Tissue-Engineered Heart Valves In Vitro
Keywords
Collagen, Chitosan, Heart, Valve
Abstract
Abstract Objective To explore the feasibility of using collagen-chitosan scaffolds to construct tissue-engineered heart valves in vitro. Methods: Observe the structural characteristics of the materials, conduct parallel rabbit subcutaneous embedding experiments, and observe the biocompatibility and biocompatibility of the materials at 4, 6, 8, and 12 weeks respectively. Degradation rate. Canine aortic wall interstitial cells and endothelial cells were planted on the chitosan collagen scaffold, their morphology was observed and the function of the cells in synthesizing collagen and prostacyclin was determined. The results showed that the collagen chitosan film had a mesh-like structure. The pore size is (103¡À17) ¦Ìm. The subcutaneous embedding experiment shows that the material has good biocompatibility and the complete degradation time is 12 weeks. The transplantation experiment shows that the cells grow well on the surface of the material and have the ability to synthesize prostacyclin (62.3¡À3.2 pg/mlvs10 .7¡À2.1 pg/ml, P<0.05). Conclusion Using collagen chitosan film as a scaffold to construct tissue engineered heart valve leaflets in vitro, the transplanted cells can not only grow on the surface of the material, but also synthesize intercellular matrix and vascular activity. material, which initially suggests that it is feasible to apply this group of materials and methods to construct tissue engineered heart valve leaflets. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Weiyong, Zhu Jinru, Li Wei, Li Ruobing, Wang Gang, Zhao Dong'e, Qiu Licheng
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