Screening And Identification Of Collagenase-Producing Strains, Fermentation Optimization And Collagenase Purification
Keywords
Screening, Purification And Fermentation Optimization Of Collagenase From Bacillus Cereus
Abstract
[Purpose] To screen out a new type of collagenase strain from the soil near a meat processing factory, and study the purification method of its collagenase by increasing its enzyme production, and further study the degradation efficiency of collagenase by this collagenase. [Method] The strain was identified through morphological characteristics, physiological and biochemical characteristics and 16SrRNA gene evolutionary tree analysis, and the fermentation conditions for enzyme production of the strain were optimized. Finally, strong anion exchange resin was used to isolate and purify the collagenase produced by the strain. [Result] The strain was identified as Bacillus cereus. The enzyme-producing fermentation conditions of this strain were optimized, and the results showed that the optimal carbon source was 2.0% glucose, the optimal nitrogen source was 1.5% tryptone, and the optimal inorganic salt was 0.005% Ca2+; the initial fermentation broth pH was 7.5, and the fermentation temperature was 37 ¡æ, under optimal conditions, the enzyme activity of the fermentation broth of this strain was (65.81¡À2.06) U/mL. Compared with (26.7¡À1.9) U/mL before optimization, the enzyme activity increased by about 1.5 times. The collagenase produced by this strain was isolated and purified, and a collagenase with a molecular weight of approximately 100 kDa and a purity greater than 90% was obtained. Its specific enzyme activity was approximately (7615.0¡À78.7) U/mg. [Conclusion] The collagenase discovered in this study has high enzymatic activity and can effectively degrade collagen into biologically active collagen peptides in a short time. It has a wide range of applications in the fields of food, medical care, health products, cosmetics and other fields. Application prospects
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Original research was done by Sun Yuanxia, Zhu Yueming, Li Xingshuo, Bai Wei, Guan Yuping, Jia Shiru
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