Development Of Human Genetically Engineered Antibodies To Type Iv Collagenase
Keywords
Type Iv Collagenase, Human Single Chain Antibody, Tumor Metastasis
Abstract
The increased expression and activity of type IV collagenase in tumor tissue is closely related to tumor metastasis. Substances that inhibit the activity of type IV collagenase can effectively inhibit or reduce tumor metastasis. Therefore, type IV collagenase has become an auxiliary tool for the diagnosis and control of malignant tumors. One of the marker molecules for transfer. Using phage antibody display technology and on the basis of human antibody library research, a type IV collagenase-specific human single-chain antibody hCo4 was successfully developed. This antibody is mainly composed of variable antibody light chains. Region and heavy chain variable region, with a molecular weight of approximately 27ku. Both ELISA and Western blotting have proven that hCo4 can specifically bind to type IV collagenase. It is the human genetically engineered antibody with the smallest molecular weight among the type IV collagenase-specific antibodies currently reported. The affinity of this antibody is the same as that of mouse single-chain antibodies prepared from hybridoma cells secreting anti-type IV collagenase monoclonal antibodies. It may become a tool to study the relationship between type IV collagenase and tumor metastasis and to be used in tumors. Immunodiagnosis and therapy.
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Original research was done by Tang Jian, Yan Xiyun, Liu Yuanyi, Tian Huasong, Tian
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