Collagen-Microcarriers Extracted From Calf Skin Are Used For Large-Scale Culture Of Adherent Cells
Keywords
Collagen, Microcarriers, Cell Culture, Bioreactor
Abstract
Because collagen has the characteristic of combining with substances such as fibronectin in culture media containing bovine serum, collagen is a widely sourced and excellent matrix that can be used to prepare cell culture media. After various forms of cell culture experiments, gt-2 microcarriers synthesized from denatured collagen can be successfully used for the adherent culture of vero, cho, bowes and fish cells. The culture scale includes static culture of different volumes, roller bottle culture, and large-scale cell culture in 1.5l and 20l imported and domestic bioreactors.
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Original research was done by Chen Yinliang, Li Yutian, Zhang Shu, Yuan Song Yaojun, Ye Nanzhang, Huaping, Yuan Tao, Ding Jianchun
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