Determination Of Serum Type ¢ô Collagen By Chemiluminescent Double-Antibody Sandwich Enzyme Immunoassay
Keywords
Type Iv Collagen, Chemiluminescence Enzyme Immunoassay, Liver Fibrosis
Abstract
Using anti-human type IV collagen polyclonal antibodies prepared from immunized rabbits and using a modified sodium periodate method and horseradish peroxidase-labeled anti-human type IV collagen monoclonal antibodies, an enhanced chemiluminescence dual assay for serum type IV collagen was established. Anti-sandwich enzyme immunoassay. The experimental results show that the method is sensitive, accurate, stable and specific. Its minimum detection limit is 30 ¦Ìg/l, the average recovery rate is 94.5%, and the intra-batch and inter-batch variation coefficients are ¡Ü6.0% and 11.6% respectively. .The reference value was measured in 60 healthy individuals and was 160 ¦Ìg/l. It correlated well with the imported Japanese kit, so it can be used as a reference alternative method for clinical serum type IV collagen radioimmunoassay.
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Original research was done by Wang Tiancheng, Yao Jiji, Liu Xiangyi, Kou Lijun
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