Optimization Of Acid-Soluble Collagen Extraction Process From Yellow River Carp Scales Using Response Surface Methodology
Keywords
Yellow River Carp Scales, Collagen, Response Surface Methodology, Extraction Process, Optimization
Abstract
Using fresh Yellow River carp scales as raw materials, on the basis of single factor experiment, response surface method Box-Behnken experimental design was used to analyze the three influencing factors that affect the extraction efficiency of acid-soluble collagen, namely acetic acid concentration, liquid-to-material ratio and extraction time. Optimize, establish and analyze the mathematical model of the relationship between various factors and the yield of acid-soluble collagen. The results show that the optimal extraction process conditions for acid-soluble collagen from Yellow River carp scales are the acetic acid concentration of the extractant 0.54mol/L, the liquid-to-material ratio 10:1 (mL/g), and the extraction time 42h. Under these conditions, the extraction of collagen The rate reaches 15.89%
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Original research was done by Kang Shuaifei, Zhu Wenxue, Wang Lingling, Xiao Feng, Qiu Yuanyuan
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