In Vitro Study Of New Plga/Collagen Composite Materials For Cartilage Regeneration
Keywords
Polylactic Acid Glycolic Acid (Plga), Collagen, Composite Materials, Cartilage Regeneration
Abstract
Objective To design and prepare new lactic-co-glycolic acid copolymer [poly(lactic-co-glycolicacid), PLGA]/collagen (collagen) composite materials, study its promoting effect on cartilage regeneration in vitro, and provide new scaffold materials for cartilage tissue engineering. . Methods The collagen porous sponge was composited with PLGA woven omentum using freeze-drying technology; the surface microstructure of the material was observed with scanning electron microscope, and the pore size was counted using image software; bovine articular chondrocytes (BAC) were isolated and cultured, and inoculated into PLGA/glue Raw materials were used to detect cell inoculation efficiency, and scanning electron microscopy was used to observe cell growth inside the material; after 1 week of in vitro culture, DNA and glycosaminoglycan (GAG) content were detected, and real-time PCR was used to detect type I collagen, type II collagen, and aggregated protein. Aggrecan mRNA expression intensity. Bovine knee articular cartilage tissue and BACs cultured in monolayer in vitro were used as controls. Results: A new PLGA/collagen composite material was successfully constructed, with a surface pore size of (136.4¡À11.8) ¦Ìm; cell seeding efficiency of 87.8%¡À1.6%; BACs grew actively on the surface and center of the material. The DNA and GAG content after 1 week of culture, ¢ò The expression intensity of collagen and aggrecan mRNA was significantly higher than that of the control group (P<0.05). Conclusion The newly designed PLGA/collagen composite material can promote cartilage regeneration in vitro and can be used as a scaffold material for cartilage tissue engineering research. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Dai Wenda, Zhang Chi, Chen Guoping, Yao Zhenjun, Dong Jian
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