Performance And Application Of Collagen Fiber-Fixed Saccharomyces Cerevisiae Bod Sensor
Keywords
Collagen Fiber, Bod, Biosensor, Immobilized Saccharomyces Cerevisiae
Abstract
Metal ion-modified collagen fiber is a new type of cell immobilization material. Using it as a microorganism immobilization carrier for sensors can increase the number of immobilized microorganisms and better maintain microbial activity. A BOD microbial sensor was prepared using metal ion zirconium (Zr)-modified collagen fiber (ZICF)-immobilized Saccharomyces cerevisiae as the response element, and the effects of different temperatures, pH, NaCl concentrations, and amounts of immobilized Saccharomyces cerevisiae on sensor performance were investigated. The results show that the sensor performs better at 29¡ãC, pH 6.0, NaCl concentration <3.5%, and 400 mg of immobilized Saccharomyces cerevisiae; under these conditions, its BOD linear response range is 2 to 200 mg/L, and the response time is 2 to 9 minutes. The immobilized Saccharomyces cerevisiae used in the sensor can be used continuously for more than 200 times, can be used stably for more than 42 days, and still maintains good activity after being stored at 4¡ãC for 7 menstrual activation. The OECD fitted wastewater is more suitable as the standard solution for the sensor than the GGA standard solution; when measuring simple organic substances, river water and simulated seawater, the results measured by this sensor method and the standard BOD5 method show good correlation. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Zhao Lei, Jin Ruoyun, He Li, Chen Shujuan, Liu Shuliang, Han Xinfeng
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