Research On The Application Of Bionic Mineralized Collagen Artificial Bone In Posterolateral Fusion Of Rabbit Spine
Keywords
Mineralized Collagen, Bone Marrow, Spinal Fusion, Osteoinduction, Rabbit
Abstract
Objective To explore the bone repair and regeneration effects of bionic mineralized collagen artificial bone and composite autologous bone marrow on the rabbit posterolateral spinal fusion model. Methods Twenty-seven 20-week-old ordinary-grade male New Zealand white rabbits were used to prepare rabbit posterolateral fusion models of L5 and L6 segments by using electric burrs to peel off the transverse processes and expose the cancellous bone. They were randomly divided into 3 groups, with 9 animals in each group. In groups A, B, and C, 1.5 mL of autogenous iliac bone, 1.5 mL (30 mm ¡Á 10 mm ¡Á 5 mm) of pure bone gold artificial bone repair material, and 1.5 mL (30 mm ¡Á 10 mm ¡Á 5 mm) bone gold artificial bone repair material combined with rabbit autologous bone marrow (mixed in a ratio of 1:1). At 4, 8, and 12 weeks after surgery, the apparent hardness of the bone grafting area was observed using the hand touch method to determine the fusion of the bone grafting; 3 animals in each group were sacrificed at each time point, vertebral body specimens were cut, and X-rays were used to observe bone defect repair and Fusion status, three-dimensional CT examination was used to evaluate whether new bone was formed in the body; specimens at each time point were sectioned for HE staining to observe new bone formation and bone defect repair and fusion. Results: Manual palpation test showed that no obvious bone graft fusion was found in each group 4 weeks after surgery; 8 weeks after surgery, there were 3 (50.0%), 2 (33.3%), and 4 (33.3%) in groups A, B, and C respectively. 66.7%) achieved bone graft fusion, and 5 (83.3%), 4 (66.7%), and 5 (83.3%) achieved bone graft fusion respectively at 12 weeks. The fusion rate of group C was similar to that of group A, and all were higher than Group B. X-ray observation showed that the fusion rate of group C at 8 and 12 weeks after surgery was higher than that of group B and similar to group A. Three-dimensional CT observation showed that the bone fusion effect of group C was better than that of group B, which was close to that of group A. HE staining showed that 12 weeks after surgery, the bone graft areas of groups A, B, and C were all covered by large areas of mature lamellar bone. The material had been completely degraded, and the edge boundary with the host bone had disappeared and was tightly integrated. Conclusion Biomimetic mineralized collagen artificial bone combined with autologous bone marrow has good osteoinductive and osteogenic guiding effects. Compared with simple biomimetic mineralized collagen artificial bone material, it has better and faster osteogenesis effect, which is close to autologous bone transplantation.
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Original research was done by Qiu Zhiye, He Zhimin, Cui Yun, Zhu Jinliang, Wang Xiumei, Hu Yanli
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