Effect Of Tilapia Skin Collagen Enzymatic Hydrolyzate On The Growth Of Hacat Cells
Keywords
Tilapia Skin, Collagen Enzymatic Hydrolyzate, Antioxidant Activity, Cell Growth, Hacat Cells
Abstract
Study the effect of tilapia skin collagen hydrolyzate hydrolyzed by different commercial enzymes on the growth of human skin keratinocytes HaCaT, and explore the relationship between the antioxidant activity of collagen hydrolyzate and its cell growth-promoting effect. The results show that among the enzymatic hydrolysates obtained by hydrolyzing tilapia skin with neutral protease (NE), alkaline protease, and trypsin, NE enzymatic hydrolysate (NEH) has significant in vitro antioxidant activity. and activity in promoting the proliferation of HaCaT cells (P£¼0.05); NEH (1~5 ku) has a significant promoting effect on the growth of HaCaT cells at a mass concentration of 25 mg/mL (P£¼0.05), and within 24 h It can significantly promote wound healing at cell scratches (healing rate (81.10¡À5.77)%, P<0.05). Through amino acid composition analysis and gel chromatography analysis, it was found that NEH (1~5 ku) mainly contains 2 peptide components. The antioxidant amino acids of NEH (1~5 ku) are phenylalanine, leucine, tyrosine, The mass fractions of lysine, arginine and histidine were significantly higher than other molecular mass peptide components (P<0.05). Studies have shown that collagen peptides with a molecular mass of 1 to 5 ku obtained through NE hydrolysis can promote the growth of HaCaT cells. This may be related to the higher mass fraction of antioxidant amino acids in the peptide components. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Xiaoling, Jiang Sufeng, Jiang Hongrui, Bai Yang, Zhao Mouming
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