Effects Of Oxidized Low-Density Lipoprotein On Collagen In Rat Cardiac Fibroblasts
Keywords
Oxidized Low-Density Lipoprotein, Cardiac Fibroblasts, Collagen, Matrix Metalloproteinases
Abstract
Objective To explore the effect of oxidized low-density lipoprotein (ox-LDL) on collagen synthesis in rat cardiac fibroblasts. Methods: Neonatal mouse cardiac fibroblasts were cultured in vitro, and ox-LDL (10, 20, 50, 100g/ml) was added for 24 hours. Cell DNA synthesis was observed by 3H-thymidine incorporation method and 3H-proline incorporation method. The synthesis of collagen was observed, the activities of matrix metalloproteinases MMP-2 and MMP-9 were measured by zymography, the protein expression levels of MMP-2 and MMP-9 were detected by Westernblot, and the mRNA expressions of MMP-2 and MMP-9 were detected by RT-PCR. The results were compared with the control group. After ox-LDL acted on cardiac fibroblasts for 24 hours, it promoted cellular DNA synthesis in a concentration-dependent manner while inhibiting cellular collagen synthesis. Ox-LDL significantly increased the activities of MMP-2 and MMP-9, but there was no significant difference in the effects among the groups. At the same time, the protein expressions of MMP-2 and MMP-9 also increased significantly, with the 100 ¦Ìg/ml group having the strongest effect. 50¦Ìg/mlox-LDL can significantly increase the mRNA expression of MMP-2 and MMP-9, and the 100¦Ìg/ml group has a stronger effect. Conclusion Ox-LDL can reduce collagen synthesis and increase collagen degradation when acting on cardiac fibroblasts, suggesting that it plays a certain role in the process of myocardial remodeling.
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Original research was done by Xiao Hua, Ji Aimin, Li Zhiliang, Song Xudong, Chen Aihua
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