Application Of High-Frequency Ultrasound In Rat Collagen-Induced Arthritis Model
Keywords
Rheumatoid Arthritis, Collagen-Induced Arthritis, Ultrasound, Synovitis
Abstract
Objective To establish a stable collagen-induced arthritis (cia) model in rats, try to use high-frequency ultrasound to observe joint lesions in cia rats in vivo, and evaluate the application value of ultrasound in small animal experimental research on RA. Methods 60 wistar rats were divided into model group (50 rats) and blank group (10 rats). In the model group, bovine type II collagen and complete Freund’s adjuvant were intradermally injected into the tail. The immunization was strengthened in the same way 14 days later. The blank group was given normal saline injection at the same time. The joint and extra-articular manifestations of mice in both groups were observed regularly. On the 45th day, ankle joint scores (AI) and high-frequency ultrasound examinations were performed on all experimental rats; the rats were sacrificed, and pathological examinations were performed on a total of 120 ankle joints of 60 rats, and compared with the AI and ultrasound results. As a result, no one in the control group became ill. A total of 100 ankle joints of 50 rats in the model group were pathologically confirmed to have synovitis in 62. Pathological sections showed typical inflammatory cell infiltration, synovial proliferation, cartilage and subchondral bone destruction, and some were accompanied by pannus formation. AI was used to diagnose 42 cases, with a detection rate of 67.8%; high-frequency ultrasound was used to diagnose 54 cases, with a detection rate of 77.4%. There was a significant difference between the two (p<0.05). Conclusion High-frequency ultrasound successfully demonstrated rheumatoid lesions in the ankle joint of cia rats. High-frequency ultrasound can non-invasively reflect joint lesions and can be used as an ideal imaging for studying small animal models of rheumatoid arthritis. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Song Haixia,Wang Quanshi
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