Effects Of Collagen Discoid Domain Receptor 2 Gene Deletion On Ovarian Function In Mice
Keywords
Extracellular Matrix, Collagen Discoid Domain Receptor 2, Corpus Luteum, Infertility
Abstract
Objective To use collagen discoid domain receptor 2 (DDR2) gene-deficient mice to study the impact of DDR2 deletion on ovarian function and explore the causes of infertility in DDR2-deficient mice. Methods ¢Ù Vaginal exfoliated cell smear combined with modified Papanicolaou staining method was used to detect the difference in estrous cycle between homozygous DDR2 gene deletion (SLIE) and wild-type (WT) mice. ¢ÚAfter ovulation induction with PMSG+HCG, the difference in the number of ovulations was counted under a stereomicroscope, and HE staining was used to observe the differences in ovarian and uterine morphology and structure between SLIE mice and WT mice after ovulation induction. Results ¢Ù Adult SLIE mice lacked an obvious estrous period, and most of them were in interestrus, lacking a regular estrous cycle. ¢Ú The ovulation induction experiment found that the number of ovulation induced in SLIE mice was significantly reduced; ¢Û In the early stage of luteinization after ovulation, the uterine wall of SLIE mice was thin and small; there was no luteinization in ovarian slices; HE staining of uterine slices showed that the endometrium was thin and glandular. The body and interstitial proliferation was not obvious, no mucus was secreted, and the secretory phase failed to enter. Conclusion DDR2 deletion leads to reduced ovarian response to gonadotropins and impaired lutein production after ovulation induction.
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Original research was done by Zhang Shuya, Chen Xin, Sang Xudong, Bu Xin, Wang Yanrong, Su Jin, Pei Xiuying
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