Effects Of Ultrafiltrate From Angelica Sinensis And Red Stilbene On The Proliferation And Collagen Secretion Of Cardiac Fibroblasts After Radiation
Keywords
Radiation-Proliferated Collagen Of Angelicae And Redstilbene Cardiac Fibroblasts
Abstract
Objective: To study the effect of ultrafiltration of Angelica sinensis and red stilbene on the proliferation and collagen secretion of cardiac fibroblasts after radiation. Methods: The differential adhesion method was used to primary isolate and culture rat cardiac fibroblasts; after intervention of angelica red stilbene ultrafiltrate or radiation irradiation of cardiac fibroblasts, thiazolyl blue (MTT) colorimetric method was used to detect cell proliferation. Linked immunosorbent assay (ELISA) was used to detect the content of type ¢ñ collagen (COL-¢ñ) secreted by cells; cardiac fibroblasts were stained with 2′, 7′-dichlorofluorescein diacetate (DCFH-DA) under flow cytometry. Detect the content of reactive oxygen species (ROS); use real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) to detect the mRNA expression levels of COL-¢ñ and transforming growth factor-¦Â (TGF-¦Â) in cardiac fibroblasts; Western blot was used to detect the expression of related proteins. Results: After low-dose radiation (0.5 Gy) irradiated cardiac fibroblasts, the increase in intracellular ROS promoted cell proliferation and collagen secretion; ultrafiltration of angelica and red stilbene could promote the transcription factor E2-related factor 2 (Nrf2) in the cells. The nuclear translocation of proteins removes ROS, ultimately inhibiting the proliferation and collagen secretion of radiation-induced cardiac fibroblasts; and ultrafiltration of angelica and red stilbene significantly inhibits the expression of COL-I and TGF-¦Â mRNA in cardiac fibroblasts. Conclusion: Ultrafiltration of angelica and red stilbene can inhibit the proliferation and collagen secretion of cardiac fibroblasts induced by low-dose radiation.
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Original research was done by Liu Hui, Song Peng, Zhang Zhaofang, Yang Yixuan, Su Haiyan
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