Comparative Analysis Of The Amino Acid Composition And C And N Stable Isotopes Of Soluble And Insoluble Collagen In Ancient Bones
Keywords
Soluble Collagen, Liquid Chromatography, C And N Stable Isotopes, Amino Acid Composition
Abstract
Extracting insoluble collagen (ISC) from human and animal bones unearthed from archaeological sites and conducting C and N stable isotope analysis is currently the main research method for ancient recipe analysis. However, for bone samples from which ISC cannot be extracted, reconstruction of ancient recipes is impossible. Previously, we successfully extracted soluble collagen (SC), the degradation product of ISC in ancient bones, for the first time through gel chromatography, and found that SC and ISC have similar C and N stable isotope ratios and can also be used in ancient bone. Recipe research. In order to further explore the mechanism of similar stable isotopes between the two, this paper selected more ancient bone samples and carried out analysis of element content, stable isotope values and amino acid composition of SC and ISC. Research results show that the total amino acid composition of SC and ISC is completely consistent with the characteristics of type I collagen. In addition, compared with ISC, the composition of 8 essential amino acids in SC is very close, with a variation range of only 0.25% (n=3); while the variation in 11 semi-essential amino acids and non-essential amino acids is slightly larger (0.90%, n= 3). Obviously, the slight difference in the content of amino acids of different properties is the main reason why the C and N stable isotopes of the two proteins are similar but not the same.
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Wang Ning, Hu Yaowu, Song Guoding, Wang Changsui
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
