Study On The Biocompatibility Of Sol-Gel Bioactive Glass And Collagen Composite Porous Scaffolds
Keywords
Bioactive Glass, Bone Marrow Mesenchymal Stem Cells, Collagen, Biocompatibility
Abstract
Abstract: In the early stage of the experiment, a freeze-drying method was used to synthesize a tissue engineering scaffold composed of sol-gel bioactive glass (BG/COL) and crude collagen fibers. In this experiment, the scaffold was co-cultured with mouse bone marrow mesenchymal stem cells (rMSCs). Evaluate the cytocompatibility of the scaffold material. The scaffold material compounded with osteoblasts was implanted subcutaneously in nude mice to explore its ectopic osteogenesis performance. The results showed that rMSCs can successfully adhere to the surface of the BG/COL porous scaffold material. , spread, and migrate into the porous scaffold. As the culture time prolongs, the amount of double-stranded DNA (ds DNA) increases, and the cell proliferation is significantly different from that of the control group. Bone marrow mesenchymal stem cells seeded on the composite material were induced and cultured The sections were stained 14 days later and showed strong positive expression of alkaline phosphatase (ALP) and calcin. The nude mice in the in vivo implantation experiment were in good health and the wounds were completely healed. There were bone tissues and blood vessels around and inside the BG/COL after 6 weeks. Generation. This proves that this new type of composite porous scaffold material has good biocompatibility, and its tissue-engineered bone combined with osteoblasts has good properties of inducing osteogenesis. Therefore, this material is ideal for use in Tissue engineering scaffold materials for bone tissue repair and regeneration.
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Original research was done by Zhou Jia’an, Jiang Xiaorui, Meng Yongchun, Zhang Xinxin, Lin Cai, Chen Xiaofeng, Han Xue
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