Effect Of Bone Marrow-Derived Mast Cells On Expression Of Type Ii Collagen And Glycosaminoglycans In Chondrocytes
Keywords
Bone Marrow-Derived Mast Cells, Chondrocytes, Type Ii Collagen, C48/80, Glycosaminoglycans
Abstract
Objective To explore the effect of bone marrow-derived mast cells (bmmcs) on the expression of type II collagen and glycosaminoglycans (gag) in chondrocytes. Methods Mouse bmmcs and articular chondrocytes were primary cultured, and the second-generation chondrocytes and bmmcs cultured for 4 weeks were planted in the transwell co-culture system at a ratio of 1:10. The cells were divided into 4 groups: chondrocyte group (control group), chondrocyte + bmmcs group (bmmcs group), chondrocyte + bmmcs + disodium cromoglycate group (dscg group), chondrocyte + bmmcs + compound48/80 group (c48 /80 group), each group has 3 multiple wells, collects and lyses chondrocytes, and then detects the expression of type II collagen in chondrocytes by ELISA and western blotting, and detects it with 1,9-dimethylmethylene blue method (dbm) Gag content in chondrocytes. Results After 24 hours of culture, there was no difference in the contents of type II collagen and gag between the bmmcs group and dscg group and the control group (p>0.05). The contents of type II collagen and gag in the c48/80 group were significantly lower than those in the control group and bmmcs group (p <0.01), the results at 48h were consistent with those at 24h. Comparing 24h and 48h, there was no difference between each group (p>0.05). Conclusion C48/80 activated bmmcs can reduce the expression of type II collagen and gag in chondrocytes.
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Original research was done by Ouyang Qingqing, Zhao Jinjun, Yang Min
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