Effects Of Sulfur Dioxide And Its Derivatives On Collagen Expression In Rat Cardiomyocytes
Keywords
So2 Sodium Bisulfite Col1A1 Col3A1 Collagen
Abstract
H9C2 cardiomyocytes were exposed to 100 ¦Ìmol¡¤L-1 sodium bisulfite for different times (3, 6, 12, 24 h). Wistar rats were used as models for whole animal exposure. The SO2 group dynamically inhaled SO2 (7 mg¡¤m-3) for 28 days, 4 hours a day; the SO2+NALC (N-acetylcysteine) group inhaled SO2 under the same conditions, and received an intraperitoneal injection of 50 mg¡¤kg-1 every other day starting from the day of SO2 exposure. (bw) NALC, the control group was inhaled with fresh air and injected with normal saline. The ROS content in rat heart tissue and H9C2 cells was measured; the mRNA of type I collagen (Col1a1) and type III collagen (Col3a1) was analyzed by fluorescence quantitative PCR and Western blot. Transcription and protein expression levels. The results show that oxidative stress caused by SO2 and its derivatives significantly increases the production of reactive oxygen species (ROS); SO2 and its derivatives cannot induce Col1a1 and Col3a1 mRNA transcription levels in rat heart tissue and H9C2 cells. significant changes, but the protein expression levels of Col1a1 and Col3a1 increased significantly; at the same time, NALC can reduce the production of ROS in cardiac tissue, effectively inhibiting the increase in the protein expression of Col1a1 and Col3a1 after SO2 inhalation. This suggests that SO2 inhalation may ultimately cause ROS through the production of Increased collagen expression
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