3D Printing Silk Fibroin-Type ¢ò Collagen Cartilage Scaffold Silk Fibroin-Type ¢ò Collagen Cartilage Scaffold Fabricated By 3D Printing Technology
Keywords
Silk Fibroin-Type ¢ò Collagen, Cartilage Scaffold, Rate-Dependent, Cell Proliferation, Cartilage Scaffold, Rate-Dependent, Cell Proliferation, Silk Fibroin-Type ¢ò Collagen
Abstract
Abstract: The macrostructure of the cartilage scaffold was designed using the three-dimensional drawing software Solidworks, and the silk fibroin-type II collagen cartilage scaffold was prepared using 3D printing technology and freeze-drying technology. The density, porosity and elastic modulus of the scaffold were tested experimentally; after chondrocytes were seeded on the scaffold, the proliferation and morphology of the cells on the scaffold were analyzed using three methods: MTT method, HE staining and scanning electron microscopy. The results show that the elastic modulus of the silk fibroin-type II collagen cartilage scaffold is rate-dependent, that is, as the strain rate increases, the elastic modulus of the scaffold increases; the density and porosity of the scaffold are (0.086 6 ¡À 0.008 4) respectively. g/cm3 and (89.3¡À3.26)%. After 7 days of culture with cells inoculated on the scaffold, the growth and proliferation of the cells accelerated; HE staining showed that the cells grew most in the surface area and least in the deep area; scanning electron microscopy showed that the scaffold pores had regular shape, good permeability, and most cells were distributed in the pores. wall surface. Abstract: The macroscopic structure of cartilage scaffold was designed by using Solidworks, and the silk fibroin-type ¢ò collagen cartilage scaffold was prepared by 3D printing technique and freeze-drying technique. The density, porosity and elastic modulus of the scaffolds were tested by experiments . The proliferation of the cells was analyzed by MTT assay, HE staining and scanning electron microscopy. The results show that the silk fibroin-type II collagen scaffold is dependent on the strain rate. The elastic modulus of scaffold increases with the increase of strain rate . The density and porosity of scaffold were(0.086 6¡À0.008 4)g/cm3 and(89.3¡À3.26)%, respectively. The cell growth and proliferation were accelerated after 7 days of inoculation. By analyzing the results of HE staining, it is found that the cells grow most in the surface area and there are the least cells in the deep region. The microscopic images by Scanning electron microscopy(SEM)reveal that the diameter of scaffold is regular and the permeability is better. The cells are mostly distributed on the surface of the spine
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Original research was done by YUAN Qing-xian, GAO Li-lan, LI Rui-xin, LIU Ying-jie, LIN Xiang-long, ZHANG Xi-zheng
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