Therapeutic Effect Of Immature Dendritic Cells On Collagen-Induced Arthritis In Mice
Keywords
Rheumatoid Arthritis Collagen-Induced Arthritis Dendritic Cells Regulatory T Cells Immune Tolerance Cytokines Rheumatoid Arthritis Collagen-Induced Arthritis Dendritic Cells Regulatory T Cells Immune Tolerance Cytokines
Abstract
Objective To explore the therapeutic effect and mechanism of immature dendritic cells (imDC) on collagen-induced arthritis (CIA) in mice. Methods Mononuclear cells derived from mouse bone marrow were isolated and cultured, and induced into immature dendritic cells (imDC) and mature dendritic cells (mDC) by cytokines and lipopolysaccharide (LPS) respectively, and flow cytometry was used. Check cell phenotypes. Thirty female BALB/c mice aged 6 to 8 weeks were selected. All mice were immunized with chicken type II collagen for the first time. On the 6th day after the initial immunization, the mice were divided into imDC group, mDC group and phosphate group according to the random number table method. In the saline buffer (PBS) group, there were 10 mice in each group, and imDC, mDC and PBS were infused through the tail vein respectively; on the 7th day after the initial immunization, the mice in the three groups were boosted. On the 21st day after boosted immunization, the joint deformation degree of the three groups of mice was observed, and their arthritis index (AI), serum anti-inflammatory factor IL-10, TGF-¦Â levels, and spleen CD4+CD25+ regulatory T cells (Treg )Proportion. Results After induction with IL-4 and GM-CSF, the expression rates of CD80, CD86, and MHC-II in bone marrow mononuclear cells were 32.3%, 25.6%, and 44.0% respectively. After induction with IL-4, GM-CSF, and LPS, The expression rates of CD80, CD86, and MHC-II were 91.5%, 90.9%, and 94.2% respectively, indicating that imDC and mDC were successfully induced and differentiated. On the 21st day after boosting immunization, the AI value of mice in the imDC group was (7.32¡À1.63) points, which was significantly better than the mDC group [(13.64¡À2.02) points] and the PBS group [(12.78¡À1.96) points] at the same time point. On the 21st day after the boosted immunization, ELISA testing showed that the serum IL-10 and TGF-¦Â and spleen CD4+CD25+Treg proportions of CIA mice in the imDC group were significantly higher than those of the mDC group and PBS group at the same time point, and the differences were statistically significant. scientific significance (P<0.01). Conclusion Immature dendritic cells can effectively inhibit the progression of CIA in mice. The mechanism may be related to the fact that immature dendritic cells can stimulate the proliferation of Treg cells by upregulating the expression of anti-inflammatory cytokines, thereby inducing immune tolerance. Objective To explore the therapeutic effects of immature dendritic cells on collagen-induced arthritis (CIA). MethodsMurine marrow-derived monocytes were isolated and cultivated with cytokines to generate immature dendritic cells (imDCs) and with lipopolysaccharide (LPS) to generate mature dendritic cells ( mDCs). The differentiation and phenotypes were confirmed with flow cytometry. Murine models of rheumatoid arthritis (RA) were established with collagen II transfusion through the caudal vein. On day 6 after first immunization, subdermal injections of imDC, mDC or PBS were administered, and the mice were divided into three groups according to the injection they had received. On day 7 a second immunization was imposed to fortify CIA. Four weeks after the first immunization the severity of CIA was evaluated using an arthritis index (AI), serum levels of IL-10 and TGF-¦Â using ELISA, as well as regulatory T cell (Treg) populations using flow cytometry. ResultsThe expression rates of CD80, CD86 and MHC-II by DCs induced with rrIL-4 and rrGM-CSF were 32.3% , 25.6% and 44.0%, and the rates by DCs induced with rrIL-4, rrGM-CSF and LPS were 91.5%, 90.9% and 94.2%, which identified the differentiation and phenotypes of the imDCs and mDCs. The aveage AI of the imDC group was 7.32¡À1.63, significantly lower than those of the mDC group (13.64¡À2.02) and the PBS group (12.78¡À1.96). The average serum concentrations of IL-10 and TGF-¦Â in the imDC group were significantly higher than in the mDC and PBS groups. The proportion of Treg in the splenocytes of the imDC group was significantly higher than in the mDC and PBS groups. ConclusionCIA was markedly ameliorated by imDC, possibly through up-regulating the expression of anti-inflammatory cytokines like IL -10 and TGF-¦Â and activating the Treg population, which could lead to immunity For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Yang Xiao, Peng Hao, Zhou Jianlin, Fang Hongsong, Chen Sen
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