Effects Of Yc-1 On Proliferation And Collagen Synthesis Of Rat Pulmonary Artery Adventitial Fibroblasts Induced By Hypoxia
Keywords
3-(5′-Hydroxymethyl-2′-Furyl)-1-Phenylindole, Hypoxia-Inducible Factor Inhibitor, Adventitial Fibroblasts, Proliferation, Collagen Synthesis, Hypoxia-Inducible Factor-1¦¡, Transformation Growth Factor-¦¢1
Abstract
Objective To initially explore the effect of hypoxia-inducible factor inhibitor 3-(5′-hydroxymethyl-2′-furyl)-1-phenylindole (YC-1) on hypoxia-induced rat pulmonary artery adventitial fibroblasts. Effects on proliferation and collagenogenesis, and possible molecular mechanisms. Methods Rat pulmonary artery fibroblasts were cultured under hypoxic conditions, and the cells were randomly divided into normoxia group, hypoxia group, and hypoxia + YC-1 (0.01, 0.05 and 0.1 mmol/L) groups. The MTT method was used to detect cell proliferation, the 3H-proline incorporation method was used to detect collagen synthesis, the Western blot method was used to detect the expression of hypoxia-inducible factor-1¦Á (HIF-1¦Á) protein, and the reverse transcription-polymerase chain reaction was used to detect transformation. Growth factor-¦Â1 (TGF-¦Â1) mRNA expression. Results The fibroblast proliferation and 3H-proline incorporation in the hypoxia group were significantly higher than those in the normoxia group (both P<0.01). Fibroblast proliferation and 3H-proline incorporation in the YC-1 group were significantly lower than those in the hypoxia group, but still higher than those in the normoxia group; the expressions of HIF-1¦Á protein and TGF-¦Â1 mRNA in the hypoxia group were significantly higher than In the normoxic group (P<0.01), YC-1 dose-dependently inhibited the expression of HIF-1¦Á protein and TGF-¦Â1 mRNA stimulated by hypoxia, among which the HIF-1¦Á protein and TGF-¦Â1 mRNA expression in the YC-1 0.1 mmol/L group The expression of mRNA was inhibited by 65% and 61% respectively (both P<0.01). Conclusion YC-1 can inhibit the proliferation and collagen synthesis of rat pulmonary artery adventitial fibroblasts induced by hypoxia in a dose-dependent manner. Its effect may be related to the down-regulation of HIF-1¦Á and TGF-¦Â1 mRNA expression. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Ren Juan, Xu Ruijuan, Zeng Qunli, Li Long, Hu Zhenhong
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