Study On The Effect And Mechanism Of Yishenjuanbi Pills On The Pathological Changes Of Ankle Joint In Rats With Type Ii Collagen-Induced Arthritis.
Keywords
Yishenjuanbi Pill Type Ii Collagen-Induced Arthritis Vascular Endothelial Growth Factor Gene Chip Pannus
Abstract
Objective To observe the therapeutic effect of Yishen Jubi Pills on rats with type II collagen-induced arthritis (CIA) and its effect on the pathological changes of ankle joint tissue, and to explore the effects of Yishen Jubi Pills on the treatment of rheumatoid arthritis (RA). Vascular endothelial growth factor (VEGF) correlation. Methods: By establishing a CIA rat model, we observed the therapeutic effect of Yishen Guanbi Pills on foot swelling in CIA rats and on the pathological damage of local tissue in the ankle joint. The expression level of VEGF in the synovial tissue was detected by immunohistochemistry. , using gene chip technology to measure the gene expression of VEGF in peripheral blood lymphocytes and liver tissue. Results: The degree of ankle joint swelling of rats in the high, medium and low dose groups of Yishenjuanbi Pills was significantly reduced, and the differences were significant compared with the model group (P<0.05, 0.01, 0.001). Compared with the model group, the high, medium and low dose groups of Yishen Juanbi Pills showed reduced synovial cell proliferation, significantly reduced synovial tissue congestion and edema, reduced vascular proliferation and the number of infiltrating inflammatory cells, significantly reduced pannus formation, and reduced fibrous tissue formation. , the degree of destruction of cartilage and bone tissue is reduced. Compared with the model group, the expression of VEGF in the synovial tissue of the high- and medium-dose Yishenjuanbi Pill groups was significantly lower (P<0.05, 0.01). The expression of VEGF in the synovial tissue of the low-dose group also showed a downward trend. The high and medium dose groups of Yishen Jubi Pills can significantly down-regulate the expression of VEGF-B gene in liver tissue (fold change>1.5). Conclusion Yishen Jubi Pills have a significant therapeutic effect on CIA rats, can significantly reduce the swelling of rat ankle joints, significantly inhibit the pathological changes of CIA rats, and can significantly down-regulate the expression of VEGF in synovial tissue and VEGF- in liver tissue. The expression of B gene can reduce the amount of VEGF in liver tissue and synovial tissue, inhibit the formation of pannus in synovial tissue, and reduce the damage to articular cartilage and bone tissue.
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Original research was done by Liu Jing, Zhang Zongpeng, Yang Dong, Wang Jingjing, Shen Xiuping, Pei Tianxian, Guo Chuanmin, Guo Jingh
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