Study On Preparation For Nano-Hydroxyapatite/Collagen Composite Study On Preparation Method Of Nano-Hydroxyapatite/Collagen Composite Material
Keywords
Tissue Engineering, Biomimic Bone, Artificial Bone, Nano-Composite Tissue Engineering, Artificial Bone, Simulated Bone, Nanocomposite Material, Hydroxyapatite/Collagen Composite Material
Abstract
The electrochemical technique was used to prepare hydroxyapatite/collagen nano-composite in situ by deposition calcium phosphate in a thick plate of demineralized bone matrix (DBM), the factors and experimental conditions, which would influence on the deposition of calcium phosphate, were investigated as well. The weight percent of inorganic ingredient was 53.9¡À3.2% determined through ash test. The distribution of inorganic ingredient (calcium phosphate, CP) in DBM was observed by microscope, the phase composition was tested through x-ray diffraction (XRD) and infrared spectroscopy (IR), the morphology and dimension of CP were investigated by transmission electron microscopy (TEM). The results indicated that calcium phosphate in DBM was non-stoichiometric apatite in the shape of rod-like and nano- dimension. The structure, component, and distribution of inorganic ingredient are closely similar to that of natural bone. It suggests that the product is nano-composite.
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Original research was done by CHEN Ji-da,WANG Yuan-liang,CAI Shao-xi,CAO Ying, Chen Jida, Wang Yuanliang, Cai Shaoxi, Cao Ying
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