Constructing Tissue Engineered Cartilage Using Stem Cells Derived From Human Placenta As Seed Cells
Keywords
Human Placenta-Derived Stem Cells, Collagen Sponge, Cartilage, Tissue Engineering
Abstract
Human placenta-derived stem cells (hPDSCs) were isolated and cultured through trypsin digestion, their biological properties were detected, and their differentiation into chondrocytes was induced under certain conditions; hPDSCs and the prepared collagen sponge scaffold material were combined in vitro Construct tissue engineering cartilage tissue, and observe its ability to form cartilage tissue after transplanting into nude mice, which provides a theoretical basis for the construction of tissue engineering cartilage tissue using hPDSCs as seed cells. The study found that hPDSCs have mesenchymal stem cell characteristics and good proliferation ability, can be continuously cultured for more than 30 generations to maintain an undifferentiated state; culturing hPDSCs in chondrocyte induction medium can differentiate into chondrocytes with biological functions. Compound hPDSCs with collagen sponge scaffold materials in the induction medium, 7 The formation of cartilage-like structures and positive expression of type ¢ò collagen can be observed after the day. In vivo transplantation experiments in nude mice confirmed that hPDSCs and collagen sponges can form cartilage tissue in vivo. Histological observation showed the formation of cartilage lacunae and the positive expression of type ¢ò collagen. .Research results show that hPDSCs have the potential to differentiate into chondrocytes, and can be combined with collagen sponge scaffold materials to construct cartilage tissue with biological functions, providing a new treatment strategy for the repair and treatment of cartilage defects in clinical work, and has broad prospects. Clinical application prospects.
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Liu Dashun, Huang Fuhui, Chai Xiangbin, Wang Binquan, Qiu Jianhua
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
