Effectsofkirenolonbovinetypeiicollagen-Inducedratlymphocytesinvivoandinvitro
Keywords
Keywords: Chinonol, Bovine Type Ii Collagen, Lymphocytes, Rheumatoid Arthritis
Abstract
Abstract: Objective To observe the immunomodulatory effect of chinonol on bovine type II collagen-specific lymphocytes in vivo and in vitro, and to explore the immunosuppressive mechanism of chinonol on antigen-specific lymphocytes. Methods Wistar rats were randomly divided into control group, model group, chinonol group and prednisolone group. Except for the control group, the collagen-induced arthritis rat model was replicated and administration started on the day of modeling. After 30 days of modeling, the spleen and draining lymph nodes were removed, and single cell suspensions were prepared. After 48 hours of in vitro culture, interferon-¦Ã (ifn-¦Ã), interleukin-10 (il-10), and il- in the culture supernatant were detected by ELISA. 4. Tumor necrosis factor-¦Á (tnf-¦Á) content; under the induction of type II collagen, normal rat lymph node cells and spleen cells were co-cultured with different concentrations of chinonol to detect collagen-induced lymphocyte proliferation and apoptosis. . Results Compared with the model group, the contents of ifn-¦Ã and tnf-¦Á in the splenocyte culture supernatant of the chinonol group were reduced (p<0.05, p<0.01), and the levels of il-10 and il-4 were increased (p<0.05 , p<0.01); the contents of ifn-¦Ã and tnf-¦Á in lymph node cell culture supernatant decreased (p<0.05, p<0.001), and the levels of il-10 and il-4 increased (p<0.05, p<0.001) ; In in vitro experiments, chinonol inhibited the proliferation of type II collagen-specific lymph node cells and promoted the apoptosis of type II collagen-specific lymph node cells and splenocytes in a dose-dependent manner. Conclusion Chinonol can inhibit the secretion of pro-inflammatory factors by type II collagen-specific lymphocytes, promote the secretion of anti-inflammatory factors by collagen-specific lymphocytes, inhibit the proliferation and promote apoptosis of collagen-specific lymphocytes, and exert immunosuppression in multiple ways. effect. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Lu Yue, Xiao Juan, Wu Zaiwang, Wang Ming, Fu Hongzheng, Chen Yingyu, Qian Ruiqin
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
