Preparation Of Collagen Enzymatic Hydrolysis Complex And Its Antifreeze Activity
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Abstract
Abstract Using the degree of hydrolysis as an indicator, the conditions for alkaline protease to hydrolyze pig skin collagen were optimized. On this basis, enzymatic complexes with different hydrolysis degrees were prepared, and their antifreeze protection activity was studied. The results showed that The enzyme concentration is 5% (w/v), the enzymatic hydrolysis temperature is 50¡ãC, the enzymatic hydrolysis time is 5 hours, the pH value is 8.0, and the collagen mass concentration is 15 mg/mL to obtain the highest degree of hydrolysis, which is the best enzymatic hydrolysis condition for pig skin collagen. .Under these conditions, enzymatic complexes with hydrolysis degrees of 4%, 6%, 8%, 10%, 13% and 16% were prepared. It was found that the enzymatic complexes with a hydrolysis degree of 10% were used at a mass concentration of The protective activity against microorganisms is highest at 1.2 mg/mL. The enzymatic complex with this degree of hydrolysis can also significantly inhibit the recrystallization of ice cream and has a high thermal hysteresis activity of 4.64¡ãC. Its molecular weight is mainly distributed in the range of less than 1 Within 000 Da
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