Exploration On The Therapeutic Mechanism Of Simvastatin In Rats With Collagen-Induced Arthritis
Keywords
Simvastatin, Arthritis, Anti-Inflammatory Effect, Tumor Necrosis Factor Alpha, Interleukin 6, Caspase-3
Abstract
Objective To use collagen-induced arthritis (cia) rats as a model to observe the effect of simvastatin on the expression of serum cytokines and synovial tissue aspartate proteolytic enzyme-3 (caspase-3), and explore the treatment of simvastatin Possible mechanisms of rheumatoid arthritis. Methods Bovine type ¢¢ collagen was used to establish the cia model. The rats (16 rats) that were successfully modeled were equally divided into the model group and the drug group. The rats that were not modeled (7 rats) were the control group. The drug group was given 2.0 mg/(kg¡¤d) by gavage, and the control and model groups were given 5 ml/(kg¡¤d) sterile water for injection. The arthritis score (ai) and toe volume of the rats were recorded once a week; on the 42nd day after the initial immunization, blood was taken from the inner canthus of the eyes and the ELISA method was used to detect serum tumor necrosis factor (tnf)-¦Á and interleukin in each group. (il)-6 level, the rats were sacrificed, and the hindlimb ankle joints were harvested for HE staining, and immunohistochemistry was used to detect the expression of caspase-3 in synovial tissue. Results The ai of the drug group and the model group began to decrease 21 days after the initial immunization, and after 35 days, the drug group was lower than the model group (all p<0.05). The toe volume of the two groups decreased slowly 14 days after the initial immunization, but was still significantly higher than that of the control group; the drug group was lower than the model group on 35 and 42 days (both p<0.05). 42 days after the initial immunization, the levels of tnf-¦Á and il-6 in the drug group were lower than those in the model group and higher than those in the control group (all p<0.05). Compared with the model group, the drug group showed significantly reduced ankle synovial tissue hyperplasia, with only a small amount of inflammatory cell infiltration, and the expression of caspase-3 was significantly increased. Conclusion Simvastatin can significantly inhibit the secretion of serum tnf-¦Á and il-6 in cia rats, upregulate the expression of caspase-3 in synovial tissue and reduce synovial tissue hyperplasia. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Wang Youlian, Yang Mingfeng, Shang Ke, Pi Hui
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