Expression Of Hnrnp-E1 And Changes In Collagen Metabolism After Acute Pulmonary Embolism In Rats
Keywords
Pulmonary Embolism, Acute, Western Blot, Semi-Quantitative Reverse Transcription-Polymerase Chain Reaction, Hnrnp-E
Abstract
Objective To study the expression changes of heterogeneous nuclear ribonucleoproteE1 (hnRNP-E1) in the lung tissue of rat acute pulmonary embolism model and its effect on collagen metabolism. Methods A rat model of acute pulmonary embolism was established. The lung tissue was removed through thoracotomy at 1, 2, 7 and 21 days after acute pulmonary embolism, and then the total RNA and total protein of the lung tissue were extracted. Normal rats were used as the control group, and semi-quantitative measurements were taken. The RT-PCR method was used to study the changes in the expression of hnRNP-E1 at the mRNA level; the Westernblot method was used to further verify the changes in the expression of hnRNP-E1 at the protein level. Northern blot method was used to analyze the expression changes of Collagen¦Á1(¢ñ)(COL1A1) and Collagen¦Á1(¢ó)(COL3A1) mRNA levels in rat lung tissue after pulmonary embolism; hydroxyproline (HYP) and Masson staining were used to observe acute pulmonary embolism 3 Collagen deposition in lung tissue after weeks. Results At different time points after acute pulmonary embolism in rats, the expression of hnRNP-E1 mRNA and protein levels increased significantly. The mRNA expression level of COL1A1 in the lung tissue of rats increased significantly after acute pulmonary embolism, but the mRNA expression level of COL3A1 did not change significantly. Both HYP analysis and Masson staining showed that collagen deposition in lung tissue increased significantly 3 weeks after acute pulmonary embolism. Conclusion The expression of hnRNP-E1 in the lung tissue of rats after acute pulmonary embolism increases, which may promote the protein expression of COL1A1 and the deposition of collagen in the lungs.
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Original research was done by Li Shengqing, Ouyang Haifeng, Liu Aru, Jian Wen, Xinyu, Ren Xinling, Zhao Feng
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