Study On The Preparation Of Polycaprolactone/Type I Collagen Tissue Engineering Meniscal Scaffold By 3D Printing And Its Physical And Chemical Properties
Keywords
Meniscal Tissue Engineering, Low-Temperature Deposition Technology, 3D Printing Technology, Scaffold Materials, Physical And Chemical Properties
Abstract
Objective To prepare polycaprolactone (PCL)/type I collagen tissue engineering meniscal scaffold (hereinafter referred to as PCL/type I collagen meniscal scaffold) by 3D printing using low-temperature deposition technology, and explore its physical and chemical properties. Methods: 15% PCL/4% type I collagen solution and 15% PCL solution were prepared, and the PCL/type I collagen meniscal scaffold and PCL meniscal scaffold were 3D printed using low-temperature deposition technology. The morphology and microstructure of the stent were observed grossly and under scanning electron microscopy, the compression modulus and tensile modulus of the stent were measured in biomechanical tests, the composition of the stent was analyzed by infrared spectroscopy, and the contact angle of the stent surface was measured; the two stents and their extracts were compared with rabbit half moon The plate cells were compositely cultured, and cell counting kit 8 (CCK-8) was used to detect cell proliferation, and normal cultured cells were used as controls; scanning electron microscopy was used to observe cell adhesion and growth in the scaffold-cell complex. Results Gross and scanning electron microscopy observations showed that both scaffolds had oriented three-dimensional microstructures and pores, but the surface of the PCL/collagen type I meniscal scaffold was rougher. In biomechanical testing, there was no statistically significant difference in the compression modulus and tensile modulus of the two stents (P>0.05). Infrared spectrum analysis showed that PCL and type I collagen were successfully mixed in the PCL/type I collagen meniscal scaffold. The surface contact angle of the PCL/type I collagen meniscal scaffold was (83.19¡À7.49)¡ã, which was significantly lower than that of the PCL meniscal scaffold (111.13¡À5.70)¡ã (t=6.638, P=0.000). CCK-8 detection showed that with the extension of culture time, the number of cells cultured in the two scaffold extracts showed an increasing trend, and there was no statistically significant difference compared with the control group (P>0.05). Scanning electron microscopy of the scaffold-cell complex showed that there were more cells adhered to the surface of the PCL/type I collagen meniscal scaffold than the PCL meniscal scaffold. Conclusion The PCL/type I collagen meniscal scaffold prepared by 3D printing using low-temperature deposition technology has excellent physical and chemical properties and no cytotoxicity, and is promising as a meniscus tissue engineering scaffold material.
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Original research was done by Liu Shuyun, Zhuo Naiqiang, Zhang Xueliang, Zhang Bin, Li Xu, Li Haojiang, Wang Zhenyong, Hao Libo, Guo Quanyi, Guo Weimin, Chen Mingxue, Gao Shuang, Xian Hai
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