Experimental Study On The Prevention Of Post-Abdominal Adhesions In Rabbits Using Polylactic Acid/Glycolic Acid Membrane
Keywords
Polylactic Acid/Glycolic Acid Membrane, Collagen Fiber, Transforming Growth Factor-¦¢1, Postoperative Adhesions In Abdominal Cavity
Abstract
To explore the preventive effect and mechanism of polylactic acid/glycolic acid (PLGA) membrane on abdominal postoperative adhesions in rabbits. 66 Japanese big-eared white rabbits were randomly divided into normal control group, model control group and PLGA membrane group. Except for the normal control group, the other groups used the multi-factor method to create rabbit abdominal postoperative adhesion models. The wounds of the PLGA membrane group were covered with PLGA membrane, while the wounds of the model control group were not treated. Blood routine, plasma liver and kidney parameters were measured at different time points. function and fibrinogen content. At 1, 2, 4, 6, and 12 weeks after surgery, the animals were sacrificed and the degree of abdominal adhesion was evaluated. Cecal tissue was taken for Masson staining and transforming growth factor-¦Â1 (TGF-¦Â1) immunohistochemistry to observe the proliferation of collagen fibers and the positive expression of TGF-¦Â1. The degree of postoperative adhesion showed that the PLGA membrane group was significantly lower than the model control group, and it had no significant impact on liver and kidney function and blood routine indicators. However, it could significantly reduce the fibrinogen content and the number of white blood cells 1 week and 2 weeks after surgery, respectively. Moreover, the surface density of collagen fibers and the total optical density of TGF-¦Â1 were significantly lower than those of the model control group. The results show that PLGA membrane can effectively prevent abdominal postoperative adhesions in rabbits, which is related to reducing the leakage of fibrinogen, inhibiting the proliferation of collagen fiber tissue and the overexpression of TGF-¦Â1
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Original research was done by Hua Fei, Sun Chaoying, Xu Jianqin, Zhu Keyan, Pan Yongming, Chen Liang, Chen Fangming, Chen Minli
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