Study On Quantifying The Degree Of Myocardial Fibrosis Caused By Canine Myocardial Ischemia-Reperfusion Using Ultrasound Backscatter Integral
Keywords
Myocardial Ischemia-Reperfusion Injury, Myocardial Fibrosis, Ultrasound Backscattering Measurement Of Connective Tissue Growth Factor Type ¢ñ Procollagen Amino Telopeptide
Abstract
Objective To evaluate the degree of myocardial fibrosis caused by canine myocardial ischemia-reperfusion by using ultrasonic backscatter measurement combined with collagen metabolites. Methods 12 adult mongrel dogs were randomly divided into 2 groups: sham operation group and ischemia-reperfusion group, with 6 dogs in each group. Two groups of hemodynamic indicators were measured. Blood was collected from the internal jugular vein after 120 minutes of reperfusion. Ultrasonic backscatter measurement technology was used to detect the integrated backscatter value (IBS) of the interventricular septum and left ventricular posterior wall and the backscatter integrated period. Change value (CVIB). Enzyme-linked immunosorbent assay was used to detect the patient’s serum connective tissue growth factor (CTGF) and type I procollagen amino-telopeptide (PINP). Masson staining was performed to observe the structural changes of collagen fibers, and immunohistochemistry was used to detect the expression of type ¢ñ collagen. Results The levels of CTGF and P¢ñNP in the ischemia-reperfusion group were higher than those in the sham operation group, and the difference was statistically significant (P<0.05). Compared with the sham operation group, the IBS of the ventricular septum and left ventricular posterior wall in the ischemia-reperfusion group was significantly increased, and CVIB was significantly decreased, and the difference was statistically significant (P<0.05). CTGF and PINP levels were positively correlated with IBS (P<0.05) and negatively correlated with CVIB (P<0.05). Masson staining showed that myocardial fibrosis increased significantly in the ischemia-reperfusion group. The expression of type ¢ñ collagen in the ischemia-reperfusion group was strongly positive. Conclusion The ultrasound backscattering parameter IBS of dogs in the ischemia-reperfusion group increased, CVIB decreased, and the levels of collagen metabolites CTGF and PINP increased. Ventricular myocardial fibrosis participated in ischemia-reperfusion injury, leading to ventricular remodeling. Ultrasound backscatter measurement combined with collagen metabolites can non-invasively detect the degree of ventricular myofibrosis in the ischemia-reperfusion stage early For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Yu Lilei, Liu Haili, Sun Juan, Liao Kai, Qian Ruping, Adila Azati
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