Effects Of Hydrogen Sulfide On Collagen Synthesis In Rat Cardiomyocytes By Down-Regulating Tgf-¦¢1/Smad2/3 Pathway
Keywords
Hydrogen Sulfide, Muscle Cells, Heart, Transforming Growth Factor ¦¢1, Smad Signaling Pathway
Abstract
Objective To explore the regulatory effect and possible mechanism of gas molecule hydrogen sulfide (H2S) on collagen synthesis in rat cardiomyocytes. Methods Rat cardiomyocytes H9C2 were randomly divided into 4 groups: normal control group (added to DMEM culture medium), sodium hydrosulfide (NaSH) group (cultured with H2S donor NaHS with a final concentration of 2¡Á10¨C4 mol/L), TGF-¦Â group (culture with a final concentration of 10 ng/ml TGF-¦Â1), TGF-¦Â + NaHS group (culture with DMEM medium containing 2¡Á10¨C4 mol/L NaHS for 30 min, and then add 10 ng/ml TGF-¦Â1 for culture). After 1 hour of culture, Western blotting was used to detect the protein expression levels of p-Smad2 and p-Smad3 in cardiomyocytes. After 72 hours of culture, immunofluorescence staining was used to observe the expression of type I collagen in cardiomyocytes. Results Compared with the normal control group, there was no significant difference in the protein expression levels of p-Smad2 and pSmad3 in cardiomyocytes in the NaHS group, while the protein expression of p-Smad2 and p-Smad3 in cardiomyocytes in the TGF-¦Â group was significantly increased, and the expression of type ¢ñ collagen was significantly enhanced. . Compared with the TGF-¦Â group, the expression of p-Smad2 and p-Smad3 proteins in cardiomyocytes in the TGF-¦Â+NaHS group was significantly reduced, and the expression of type ¢ñ collagen was significantly weakened. Conclusion H2S can inhibit collagen synthesis in cardiomyocytes, and its mechanism may be related to blocking the TGF-¦Â1/Smad2/3 signal transduction pathway.
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Liu Baichuan, Sun Lili, Wang Haiying, Sun Lujing, Zhao Xiaodong, Zhao Bin
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
