Scl1 Of M41-Type Group A Streptococcus Binds Low-Density Lipoprotein
Keywords
Group A Streptococcus, Streptococcal Collagen-Like Protein 1, Low-Density Lipoprotein Keywords: Group A Streptococcus, Type ¢ñ Collagen-Like Protein, Low-Density Lipoprotein
Abstract
Abstract: Objective] To study the interaction of streptococcal collagen-like protein 1 (Scl1) of M41-type group A streptococcus (GAS) ATCC12373 with low-density lipoprotein (LDL). Methods] We cloned, expressed and purified the recombinant proteins rScl1 and its V region rScl1-V, designated as C176 and C176V, derived from Scl1.41 of M41-type GAS. The binding of rScl1 to LDL was detected with affinity chromatography-binding assay, Western blot analysis and enzyme-linked immunosorbent assay ( ELISA), and whole cell binding assays were used to detect the interactions of whole GAS cells with LDL. Results] The results demonstrated that C176 and C176V could specifically bind purified LDL, and M41-type GAS cells expressing native Scl1 could bind LDL whereas M6 -type GAS could not. Conclusion] The Scl1 of M41-type GAS specifically binds LDL.
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Original research was done by Chunwei Liang, Ruidong Zhao, Yuming Gao, Runlin Han, Liang Chunwei, Zhao Ruidong, Gao Yumin, Han Runlin
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