The Mechanism Of Action Of Natural Antioxidant Ergothioneine In Protecting Dna And Protein From Oxidative Damage Caused By Copper
Keywords
Ergothioneine, Copper Ion, Ergothioneine-Copper Complex, Dna, Strand Break, Protein Carbonylation
Abstract
Ergothioneine is a natural amino acid analog that usually exists at millimolar concentrations in biological tissues and body fluids. However, the biological functions and effects of ergothioneine are not completely clear. We have no idea about the effects of ergothioneine on The impact of oxidative damage to DNA and proteins caused by copper and its possible role were discussed in depth. Two copper-containing reaction systems, Cu(II)/ascorbic acid system and Cu(II)/H2O2 system, were used in the study. Oxidative damage to DNA and bovine serum albumin was detected through DNA chain breaks and protein carbonylation respectively. The results showed that in both copper-containing reaction systems, ergothioneine could significantly protect DNA and proteins from oxidation. Damage, and this protective effect has a dose-dependent effect. In contrast, classic hydroxyl radical scavengers (such as DMSO and mannitol) can only provide a very weak protective effect despite concentrations as high as 100mmol/L. In addition, In the study, it was found through ultraviolet-visible and low-temperature electron spin resonance analysis methods that ergothioneine can significantly inhibit the copper-catalyzed oxidation process of ascorbic acid, and can also effectively compete with histidine and 1,10-phenanthroline It complexes monovalent rather than divalent copper ions. From the above research results, we know that ergothioneine is a natural sulfur-containing antioxidant that can form an ergothioneine-copper complex that does not have redox activity. form, thereby effectively inhibiting oxidative damage to biological macromolecules caused by metallic copper ions.
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Originate research is done by Zhu Benzhan, Mao Li, Fan Ruimei, Zhu Junge DOI: 10.1360/972011-785, pp. 2283-2288
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Ergothionine (L-(+)-Ergothioneine, EGT) represents the sole natural 2-thioimidazole amino acid. This compound possesses the unique ability to permeate cell mitochondria, directly engaging in antioxidant activity at the cellular core. Furthermore, its application extends across various medical procedures, including organ transplantation, cellular preservation, and ophthalmic surgery, underscoring its significant value to human health.
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【English Name】:L-Ergothioneine
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L-(+)-Ergothioneine (Food grade)
Ergothionine (L-(+)-Ergothioneine, EGT) is distinguished as the exclusive natural 2-thioimidazole amino acid. Notably, EGT exhibits an absorption wavelength akin to DNA within the ultraviolet (UV) spectrum, effectively safeguarding against UV-induced damage. Moreover, EGT’s chemical stability allows for its accumulation in the skin cells, offering protection without toxicity.
【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
【Formula】：C9H15N3O2S
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Ergothionine (L-(+)-Ergothioneine, EGT) is the sole natural 2-thioimidazole amino acid. EGT possesses an absorption wavelength comparable to that of DNA within the ultraviolet (UV) absorption spectrum, enabling effective protection against UV harm. Additionally, the chemical stability of EGT facilitates its accumulation in the abdominal skin cells, while maintaining non-toxic characteristics.
【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
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Ergothionine (L-(+)-Ergothioneine, EGT) stands as the exclusive natural 2-thioimidazole amino acid. It features an absorption wavelength akin to DNA within the ultraviolet (UV) spectrum, thereby offering effective defense against UV-induced damage. Moreover, EGT exhibits potent antioxidant properties, capable of suppressing the genesis of diverse oxidative active agents. Its broad application spans functional skincare products, functional foods, organ transplantation, cellular preservation, and eye surgery, among other therapeutic procedures.
【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
【Formula】：C9H15N3O2S
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Ergothionine (L-(+)-Ergothioneine, EGT) is uniquely identified as the sole natural 2-thioimidazole amino acid. Possessing an absorption wavelength comparable to DNA within the ultraviolet (UV) spectrum, EGT is adept at providing effective protection against UV degradation. Additionally, EGT is characterized by a robust antioxidant capability, inhibiting the development of a wide range of oxidative active compounds. This has led to its extensive application in functional skincare products, functional foods, organ transplantation, cell preservation, ocular surgery, and various other therapeutic practices.
【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
【Formula】：C9H15N3O2S
【Specification】： 99.5% Powder
【Package】：10mg
【Function】：Bioassay, content or potency determination of antibiotics or biochemical drugs.
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