Effects Of Mn, Zn And Fe On The Contents Of Lysergic Acid Amide And Ergonovine In The Endophytic Fungal Symbionts Of Drunk Horseweed
Keywords
Drunk Horse Grass, Endophytic Fungus, Lysergic Acid Amide, Ergometrine, Mn, Zn, Fe
Abstract
This study used greenhouse sand cultivation experiments to examine the effects of different concentrations of Mn, Zn and Fe treatments on the contents of lysergic acid amide and ergonovine in the endophytic fungus symbiont of Drunk Horsegrass, in order to clarify the content of the three trace elements required for the symbiont to produce alkali. optimal concentration range. The results show that: (1) Under short-term (3 weeks) treatment, the contents of symbionts lysergic acid amide and ergonovine reached the maximum when the Mn2+ concentration was 5.0 mmol·L-1, and were significantly higher than the rest of the Mn2+ concentration gradient; Under longer-term treatment (6, 9 weeks), the lysergic acid amide content was the largest when the Mn2+ concentration was 5.0 mmol·L-1, but the increase was not obvious. Ergonovine accumulated under the control (0.01 mmol·L-1Mn2+) treatment. The amount reached the maximum and was significantly higher than that of the other treatments. (2) When the treatment time was 3, 6 and 9 weeks, the symbiont lysergic acid content reached the maximum value at 7.0 mmol·L-1 in the Zn2+ treatment, and the content in the 3-week treatment was significantly higher than that in the other treatment times; its ergot New base reached the maximum value when the Zn2+ concentration was 21.0 mmol·L-1 under the three treatment times. (3) Under different treatment times, the content of symbiont lysergic acid reached the maximum value at Fe concentration of 6.0-12.0 mmol·L-1, and the content at 3 weeks was significantly higher than that at other treatment times; ergonovine The content reached the maximum value at the Fe concentration of 0 mmol·L-1 in the 3rd and 6th weeks, and reached the maximum value at the Fe concentration of 0.03 mmol·L-1 in the 9th week. Studies have shown that high concentrations of Mn, Zn and Fe elements for a short period of time are beneficial to stimulating the accumulation of two types of ergot alkaloids in the endophytic fungal symbiont of Drunken Horsegrass. This can be improved by controlling the concentrations of the three trace elements and the growth time of the symbiont. The contents of the two ergot alkaloids provide more precise external conditions for extracting higher concentrations of lysergic acid amide and ergonovine as clinical agents or biopesticides in the future.
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Originate research is done by Wan Zhiwen, Feng Jiangrong, Wang Ping, Li Chunjie
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