Angiotensin ¢ò Induces The Synthesis Of Type ¢ñ Collagen In Passively Sensitized Human Airway Smooth Muscle Cells
Keywords
Human Airway Smooth Muscle Cells, Passive Sensitization, Angiotensin ¢ò, Type ¢ñ Collagen
Abstract
Objective To explore the effect of angiotensin ¢ò (Ang ¢ò) and its type ¢ñ receptor (Angiotensin Type 1 Receptor, AT1R) antagonist Losartan (Losartan) on the synthesis of type ¢ñ collagen in passively sensitized human airway smooth muscle cells (HASMCs). Methods HASMCs were cultured in vitro, and the cells were divided into 4 groups according to treatment factors: ¢Ù Passive sensitization group (10% asthma serum); ¢Ú Passive sensitization + Ang¢ò group (10% asthma serum + 10-7mol/LAng¢ò); ¢Û Passive sensitization group Allergy+Losartan group (10% asthma serum+10-6mol/LLosartan); ¢ÜPassive sensitization+Ang¢ò+Losartan group (10% asthma serum+10-7mol/LAng¢ò+10-6mol/LLosartan). Immunofluorescence staining was used to identify HASMCs, fluorescence quantitative PCR was used to detect type I collagen mRNA expression, and ELISA was used to detect type I collagen secretion. Results After 10-7 mol/l Ang¢ò acted on passively sensitized HASMCs for 24 hours, the expression of type I collagen mRNA and protein increased significantly compared with the passive sensitization group (P<0.01). In the presence of Losartan, the promoting effect of Ang II on the expression of type I collagen mRNA and protein in passively sensitized HASMCs was significantly inhibited (P<0.01). Conclusion Ang¢ò can promote the secretion of type I collagen in passively sensitized HASMCs, possibly by binding to AT1R. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Shen Bin, Cheng Yuanxiong, Li Ning, Niu Yi, Xie Haojun, Huo Yating
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
