Animal Experimental Study On Collagen-Hydroxyapatite Composite Membrane Guiding Bone Tissue Regeneration
Keywords
Guide Bone Tissue Regeneration, Collagen, Hydroxyapatite, Bone Defects
Abstract
Objective To explore the effect of self-made collagen-hydroxyapatite (COL-HA) composite membrane in repairing skull defects in SD rats. Methods Four defects were prepared on the cranial bones of 24 SD rats. The left and right defect areas were covered with COL-HA composite single-layer dense membrane (Group 2) and COL-HA composite double-layer membrane (Group 3) respectively. Group), the frontal bone defect area was retained as a blank control (Group 1), and the occipital bone defect area was covered with Bio-Gide membrane as a comparison (Group 4). Six rats were sacrificed at 2, 4, 8, and 12 weeks after surgery, and materials were collected for visual observation, X-ray examination, tissue section observation, and determination of new bone mass. The results were subjected to generalized linear model/factorial design variance analysis and LSD- t-test. Results Starting from 2 weeks after surgery, except for group 1, a small amount of new bone could be seen in the other three groups. At 12 weeks, the defect area in the three groups was closed by opaque hard tissue, and some decomposed membrane fragments could be seen. X-rays showed that at 12 weeks after surgery, the repaired bone density in the defect areas of groups 3 and 4 was close to that of the original bone, while that of group 2 was slightly lower. Analysis of new bone mass showed that the new bone mass in group 4 was greater than the other three groups in the early postoperative period. There was no statistical difference in the bone formation mass between group 4, group 3, and group 2 at 12 weeks after surgery. Conclusion COL-HA composite membrane can guide rat skull tissue regeneration, and the osteogenic effect of the composite double-layer membrane is better than that of the composite single-layer dense membrane, and its structure is more conducive to the attachment and growth of osteoblasts.
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Original research was done by Jin Qiong, Wang Xiaomin, Wang Xiaofei, Li Xudong, Ma Jianfeng
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