Assembly Of Heparin And Iv Collagen On Titanium Surface And The Effects Of Different Assembly Layers On Endothelial Progenitor Cells
Keywords
Titanium, Number Of Assembly Layers, Heparin, Type Iv Collagen, Cytocompatibility
Abstract
This article used layer-by-layer self-assembly technology to alternately assemble oppositely charged heparin and type IV collagen onto the surface of the material, and studied the effects on endothelial progenitor cells before and after assembly and with different numbers of assembly layers. After titanium is activated by alkali, hydroxyl groups are added to the surface, which combines with the carboxyl groups in polylysine to form a positively charged amino surface on the material. Then, heparin and type IV collagen with heterogeneous charges are alternately added to the material. Multiple self-assembled layers are formed on the surface. Fourier transform infrared grazing was used to detect changes in the surface groups of the material after each step of treatment, toluidine blue was used to quantitatively characterize the amount of heparin assembly on the surface of the material, atomic force microscopy was used to observe the surface morphology changes of the material before and after assembly, and the assembly process was tracked by measuring the water contact angle. Changes in the hydrophilic and hydrophobic properties of the material surface. The results showed that heparin and type IV collagen were successfully assembled on the material surface. The modified surface had more adherent endothelial progenitor cells than the titanium surface. As the number of assembly layers increased, the number of cells adhering to the material surface also gradually increased, and Its growth and proliferation gradually increase
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Original research was done by Deng Kun, Chen Junying
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