Blp Tolerance Enhances Macrophage Phagocytosis Through Upregulation Of Marco
Keywords
Collagen-Like Structure Macrophage Receptor^Macrophage^Phagocytosis^Bacterial Lipoprotein^Tolerance
Abstract
Abstract Purpose: To explore the role of macrophage receptor with collagen-like structure (MARCO) in the process of enhancing bacterial lipoprotein (BLP) resistance to macrophage phagocytosis. Methods: Isolate, differentiate and culture bone marrow-derived macrophages (BMDMs), and prepare BLP-resistant cells; use flow cytometry and fluorescence microscopy to detect and compare the ability of BLP-resistant and non-tolerant cells to phagocytose bacteria. Flow cytometry and qPCR were used to detect the effect of BLP tolerance on MARCO protein and mRNA expression; small interfering RNA was further used to downregulate MARCO expression, and the effect of MARCO on the phagocytosis ability of BLP-resistant macrophages was explored through flow cytometry and fluorescence technology. Results: The amount of phagocytosis of bacteria by BLP-tolerant macrophages was significantly higher than that of non-tolerant macrophages (P<0.05); at the same time, the expression level of MARCO protein on the membrane surface of BLP-tolerant macrophages was significantly higher than that of non-tolerant cells, and in It further increased after bacterial stimulation, and the changes in MARCO's mRNA levels were consistent with changes in protein levels; after down-regulating MARCO expression, the phagocytosis ability of BLP-resistant macrophages significantly decreased, suggesting that the enhanced phagocytosis ability of BLP-resistant macrophages is related to the macrophage membrane. Increased expression of surface MARCO protein. Conclusion: BLP tolerance enhances the phagocytosis of bacteria by upregulating the expression of MARCO protein on the surface of macrophage membranes. Service Recommend this article to friends Add to my bookshelf Add to citation manager Email Alert RSS Author related articles Xi Dalin Xiang Jing Cai Junwei Zhao Shuqi Ji Jingjing Li Yue Su Ting Jiang Yong Liu Jinghua Service author related articles For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Xi Dalin, Liu Jinghua, Ji Jingjing, Jiang Yong, Li Yue, Su Ting, Cai Junwei, Zhao Shuqi, Xiang Jing
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