Collagen Research

Keywords

Silk Fibroin, Collagen, Hydroxyapatite, Mc3T3-E1 Cells, Mechanical Loading

Abstract

Objective To observe the effect of dynamic mechanical loading on the proliferation, differentiation and osteogenesis-specific gene expression of MC3T3-E1 cells in the three-dimensional biomimetic composite scaffold material prepared by low-temperature 3D printing combined with freeze-drying method. Methods Silk fibroin, type I collagen, and hydroxyapatite were mixed at a mass ratio of 3:9:2, and a three-dimensional biomimetic composite scaffold was prepared using low-temperature 3D printing combined with freeze-drying technology. The appearance of the scaffold was generally observed, and the pore size of the scaffold was observed with Micro-CT. and porosity, and determine the water expansion rate, stress, strain, and elastic modulus. MC3T3-E1 cells were seeded into a three-dimensional biomimetic composite scaffold and randomly divided into two groups: the experimental group underwent dynamic mechanical loading once a day, 15 minutes each time, frequency 1 Hz, and strain 3 500 ¦Ì¦Å during culture; the control group received no mechanical loading. After 7 and 14 days of culture, HE staining and scanning electron microscopy were performed on the cell-scaffold complex to observe the cell growth on the scaffold. Western blot and real-time fluorescence quantitative PCR were used to detect type I collagen, BMP-2, and osteocalcin (OCN). ) protein and mRNA expression. As a result, the three-dimensional bionic composite scaffold prepared was a white cubic grid. Micro-CT examination showed that the interior of the scaffold material had a pore network structure with good pore connectivity; the macropore diameter was (506.37¡À18.63) ¦Ìm and the micropore diameter was (62.14¡À 17.35) ¦Ìm, the porosity is 97.70%¡À1.37%, and the water expansion rate is 1341.97%¡À64.41%. Mechanical testing shows that when the stent is compressed to 10%, the compression displacement of the stent is (0.376¡À0.004) mm, the compressive stress is (0.016¡À0.002) MPa, and the elastic modulus is (162.418¡À18.754) kPa. After 7 and 14 days of composite culture, HE staining and scanning electron microscopy of both groups showed that cells grew inside the scaffold, mainly distributed around the pore walls of the scaffold. The cells in the experimental group increased compared with the control group, and the cells changed from spindle-shaped to flat. Except for the difference in cell count between the two groups at 14 days under 200x microscope (t=¨C2.024, P=0.080), the cell numbers of the experimental groups at each time point under different magnifications (40, 100, and 400x) were all the same. Significantly higher than the control group (P<0.05). Real-time fluorescence quantitative PCR detection showed that the relative expression of type I collagen and OCN mRNA in the experimental group was significantly higher than that in the control group at 7 and 14 days of culture (P<0.05), but there was no statistical difference in the relative expression of BMP-2 mRNA between the experimental group and the control group. scientific significance (P>0.05). Western blot analysis showed that the relative expression levels of type I collagen, BMP-2, and OCN proteins in the experimental group were significantly higher than those in the control group (P<0.05) at 7 and 14 days of culture. Conclusion After dynamic mechanical loading, the expression of BMP-2, type I collagen, and OCN in MC3T3-E1 cells seeded on the three-dimensional biomimetic composite scaffold was significantly increased, indicating that appropriate mechanical loading is conducive to the differentiation of MC3T3-E1 cells into osteoblasts. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Yingjie, Zhang Xizheng, Li Hui, Li Ruixin, Cheng Wei, Yuan Qingxian  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Ordered Materials, Spinal Cord Injury, Collagen Binding Domain-Brain Derived Neurotrophic Factor, Bbb Score, Inclined Plane Score, Uniaxial Guidance Collagen Scaffolds

Abstract

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by CHEN Lei, LIU Dongxiao, LI Kaiming, SONG Xinqiang, ZENG Xiansi, JIANG Lijie

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Amniotic Membrane, Bone And Bone Tissue, Biomedical Engineering, Collagen Type I, Alkaline Phosphatase

Abstract

? Purpose: To initially explore the potential of human amniotic membrane stromal cells (hamc) as seed cells for bone tissue engineering. Methods: hamc was isolated and cultured, and the third generation hamc was induced into osteogenesis in DMEM culture medium containing dexamethasone (0.1 ¦Ìmol/l), vitamin C (50 mg/l) and ¦Â-glycerol sodium phosphate (10 mmol/l). After culturing for 1 week, alizarin red staining was performed to count the number of calcified nodules, and immunofluorescence cytochemical staining was performed to detect the expression of type I collagen and alkaline phosphatase. Immunohistochemical staining was used to detect the expression of fasl in amniotic membrane and hamc. Results: After osteogenic induction of hamc, obvious calcified nodules were formed, with an average of 18 per well. The cells in the calcified nodules expressed positive expression of type I collagen and alkaline phosphatase. The positive expression of fasl can be detected in cells in human amniotic membrane tissue and in hamc cultured in vitro. Conclusion: hamc has the potential to become an ideal seed cell for bone tissue engineering.

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Mei Fang, Tang Junmin, Zhong Jinyan, Qi Weihong, Tang Yan, Li Feng

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Sclera,Collagen,Fibrils,Fibroblasts,Elastic,Modulus

Abstract

Objective To explore the changes in morphological structure and biomechanical properties of rabbit scleral tissue at different ages. Methods: New Zealand white rabbits aged 1, 2, and 3 months were selected, the eyeballs were removed, and the diameter and axial length of the eyeballs were measured. The scleral tissue was obtained, and the thickness of the scleral tissue was measured. After a part of the scleral tissue was fixed, HE staining was performed to observe the scleral structure. After a part of the scleral tissue was fixed, the scleral structure was observed. The collagen fibrils were observed through an electron microscope, and another part of the scleral tissue was made into a scleral strip, and the elastic modulus of the sclera was measured using an Instron 5544 testing machine. Results: Eyeball diameter, axial length and scleral thickness increased with age. The elastic modulus of scleral tissue increases with age. The number of scleral fibroblasts in the scleral tissue decreases with age, and the fiber bundles increase and become thicker. The diameter of collagen fibrils increases with age. Conclusion During the postembryonic growth of sclera, the structures of the eyeball and sclera continue to change, collagen fibers continue to increase and thicken, and the biomechanical properties of scleral tissue also improve. Understanding the mechanism of postembryonic growth and development of sclera can provide guidance for the treatment of scleral-related diseases. Provide theoretical guidance for prevention and treatment. Objective To investigate the changes of morphological structures and biomechanical properties of scleral tissues in rabbits at different month ages. Methods The eyeballs of 1, 2 and 3 month-old New Zealand white rabbits were obtained for measuring the diameter and axial length, and the thickness of scleral tissues. Part of the scleral tissues was used to observe scleral structures with HE staining, some other part was used to observe collagen fibrils by electron microscope, and the left part were cut into strips and used to test the elastic modulus of the sclera on Instron 5544 system. Results The diameter, axial length of eyeballs and the thickness of the sclera were increased with month age. The elastic modulus of scleral tissues was also increased with month age. The numbers of scleral fibroblasts were decreased and the numbers of fiber bundles were increased with month age. The diameters of collagen fibrils were increased with month age. Conclusions In the post-embryonic stages, the structures of eyeball and sclera are changed continually, with growing numbers of thicker collagen fibers, and the biomechanical properties of scleral tissues are improved correspondingly. The mechanism of post-embryonic development in sclera is further explained in the study, which can provide theoretical guidance for prevention of sclera-related diseases.

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Hao Shuang, Deng Aijun, Wang Guohui

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Microrna-, Rheumatoid Arthritis, Cadherin, Tumor Necrosis Factor-¦¡, Type Ii Collagen

Abstract

Objective To explore the effect of miR-144 overexpression lentivirus on the pathological process of synovial tissue in arthritis model mice and its possible mechanism. Methods: Type II collagen emulsion was injected subcutaneously into the tail base of BALB/c mice to construct a mouse model of collagen-induced arthritis (CIA), and then a lentivirus with high expression of miRNA-144 was injected into the tail vein. Then, immunohistochemistry was performed on the synovial tissue of the model mice to detect the expression and distribution of Cadherin-11 and TNF-¦Á, and HE staining was used to detect the pathological changes in the synovial tissue. Results The synovial tissue of the model mice showed similar characteristics to the pathological process of rheumatoid arthritis. The inflammatory response of the synovial tissue of the model mice treated with lentivirus with high expression of miRNA-144 was compared with that of the saline and empty lentivirus treated groups. The expression of TNF-¦Á was significantly reduced compared with the normal saline and empty vector lentivirus treatment groups (P<0.05). Further detection of the expression of Cadherin-11 was also significantly reduced (P<0.05). Conclusion Increased miRNA-144 in the body can attenuate or inhibit the inflammatory response of synovial tissue in arthritis model mice and downregulate the expression of TNF-¦Á, and this effect may be due to the inhibition of Cadherin-11 in synovial cells by miRNA-144. effect. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Yan Yanfei, Kang Fei, Yang Bo, Miao Ying, Dong Shiwu  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Group A Streptococcus, Streptococcal Collagen-Like Protein 1, Low-Density Lipoprotein Keywords: Group A Streptococcus, Type ¢ñ Collagen-Like Protein, Low-Density Lipoprotein

Abstract

Abstract: Objective] To study the interaction of streptococcal collagen-like protein 1 (Scl1) of M41-type group A streptococcus (GAS) ATCC12373 with low-density lipoprotein (LDL). Methods] We cloned, expressed and purified the recombinant proteins rScl1 and its V region rScl1-V, designated as C176 and C176V, derived from Scl1.41 of M41-type GAS. The binding of rScl1 to LDL was detected with affinity chromatography-binding assay, Western blot analysis and enzyme-linked immunosorbent assay ( ELISA), and whole cell binding assays were used to detect the interactions of whole GAS cells with LDL. Results] The results demonstrated that C176 and C176V could specifically bind purified LDL, and M41-type GAS cells expressing native Scl1 could bind LDL whereas M6 -type GAS could not. Conclusion] The Scl1 of M41-type GAS specifically binds LDL.

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Chunwei Liang, Ruidong Zhao, Yuming Gao, Runlin Han, Liang Chunwei, Zhao Ruidong, Gao Yumin, Han Runlin

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Portal Hypertension/Schistosomiasis, Vascular Smooth Muscle Cells, Hypoxia-Inducible Factor, Type I Collagen

Abstract

Abstract Objective To observe the expression of hypoxia-inducible factor (HIF)-1¦Á in the portal vein of rabbits with schistosomiasis and explore its significance in the activation of smooth muscle cells (SMC). Methods The portal hypertension model was established by infecting rabbits with schistosomiasis cercariae through skin application. Western blot and immunohistochemistry were used to detect the expression levels of HIF1¦Á and type I collagen at 150 days. At the same time, optical microscopy and transmission electron microscopy were used to compare the media thickness and SMC ultrastructure of the portal vein of rabbits in each group. The results were consistent with those of healthy rabbit portal veins. Comparing tissues, the expression of HIF-1¦Á in schistosomiasis rabbits was significantly increased (integrated absorbance was 6.45¡À2.14 and 3.27¡À1.58, respectively, P<0.01), and the content of type I collagen was significantly increased (integrated absorbance was 141.3¡À28.21 and 48.53¡À12.22, respectively. P<0.01). In the model group, portal vein SMC significantly proliferated, transformed into synthetic fibroblasts, and the tunica media thickened (118.29¡À36.52) ¦Ìm. Conclusion HIF-1¦Á increased significantly in the portal vein of rabbits with schistosomiasis, which may promote SMC activation and participate in the portal vein Hypertensive vasculopathy formation For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Yang Zhen, Zang Yunjin, Zou Weilong, Chen Xinguo  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

Objective To explore the effect of cyclosporine A (CSA) on the proliferation and collagen synthesis of cardiac fibroblasts (CFS) induced by vasopressin (AVP). Methods: Neonatal Sprague-Dawley rat CFs were cultured using trypsin digestion method. Cells cultured in serum-free medium for more than 24 hours were given different concentrations of CSA based on AVP stimulation. The cell number was determined by the mtt method, the cell cycle was analyzed by flow cytometry, the hydroxyproline content of the cell culture supernatant was determined by the hydroxyproline colorimetric method, and the calcineurin (can) activity was measured by a spectrophotometer. Results Cyclosporine A dose-dependently inhibited the increase in the number of cardiac fibroblasts induced by AVP. The inhibition rates of 0.05, 0.5 and 5 ¦Ìmol¡¤l-1 CSA on the MTT absorbance value of cfs were 12%, 24% and 24% respectively. 29% (both p<0.05); cell cycle analysis showed that 0.5¦Ìmol¡¤l-1csa could reduce the S phase percentage and proliferation index induced by avp (p<0.05); 0.5 and 5¦Ìmol¡¤l-1csa could reduce the cell culture supernatant Liquid hydroxyproline content, inhibition rates were 29% and 33% respectively, with statistical differences (all p<0.05); csa can completely block avp-induced can activity in cfs cells, while csa has a negative effect on can activity in the basal state and cell viability were not significantly affected. Conclusion CSA inhibits AVP-induced CFS proliferation and collagen synthesis by blocking the CAN signaling pathway, inhibits the process of myocardial fibrosis, and provides a new therapeutic target for the prevention and treatment of myocardial fibrosis. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Shang Fujun, Zhao Lianyou, Zheng Qiangsun, Wang Jiepin  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Liver Fibrosis, Cucumber Aroma, Collagen Fiber, Transforming Growth Factor ¦¢1, ¦¡-Smooth Muscle Actin

Abstract

? Objective To study the antagonistic effect of cucumber aroma on liver fibrosis in rats. Methods 48 rats were randomly divided into normal control group, model group, normal saline group and cucumber fragrance intervention group. A fully automatic biochemical analyzer was used to detect alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline Phosphatase (ALP) and hematoxylin-eosin (HE) staining were used to observe the microstructure of the liver, and immunohistological staining was performed to detect type I and III collagen, ¦Á-smooth muscle actin (¦Á-SMA) and transforming growth factor ¦Â1 (TGF- ¦Â1). Results The ALT, AST and ALP of the cucumber aroma intervention group were (70.8¡À12.3), (112.3¡À26.4) and (169.1¡À21.2) U/L respectively, which were significantly lower than those of the model group (P<0.01); the cucumber aroma intervention group There was no statistically significant difference in the expression of liver type ¢ñ, ¢ó collagen, ¦Á-SMA and TGF-¦Â1 compared with the normal control (P>0.05). However, compared with the model group, the expression was significantly enhanced, and the differences were statistically significant (P< 0.01). Conclusion Cucumber aroma extract can intervene in the process of liver fibrosis and significantly reduce the area of fibrotic tissue. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Li Xianhui, Li Chunyan  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Composite, Scaffold, Cell, Biomineralization, Regulation

Abstract

The preparation of biomimetic composite scaffolds using tissue engineering strategies is an emerging method for repairing bone tissue defects. In this study, a new biomimetic composite scaffold was prepared by mixing cross-linked bioactive glass, collagen and phosphoserine. The scaffold has the same characteristics as natural Similar components to bone tissue. In vitro experiments show that the composite scaffold is mineralized in the culture medium without MC3T3-E1 cells to produce octahedral crystals. The mineralization products during the co-culture with the composite scaffold and cells have different morphology and composition. Consistent. This octahedral crystal eventually evolved into plate-like hydroxyapatite. The extracellular matrix-like components of the scaffold have a significant regulatory effect on mineralization. In vivo animal experiments confirmed that the composite scaffold has excellent bone repair properties.

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Yang Chunrong, Wang Yingjun, Chen Xiaofeng

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.