Collagen Research

Keywords

Collagen-Induced Arthritis, Rat, Iguratimod, Methotrexate

Abstract

Objective To preliminarily explore the expression of RANKL and M-CSF in the joint synovium of CIA rats and the intervention effects of T-614 and MTX. Methods Rats were immunized with collagen emulsion mixed with chicken type II collagen and complete Freund’s adjuvant to establish a CIA rat model. CIA rats were randomly divided into model control group, T-614 group, MTX group and T-614+MTX. Group. Rats in the drug intervention group were treated with T-614, MTX and T-614 combined with MTX for 3 weeks respectively. Arthritis index (AI) score was used to evaluate the degree of arthritis, HE staining was used to observe the morphological changes of ankle joint tissue, X-ray films were used to observe bone erosion, high-frequency ultrasound was used to observe ankle joints and measure synovial thickness, and Westernblot method was used to detect synovial RANKL. and M-CSF expression. Results Compared with the normal control group, the AI score, pathology score, X-ray score, membrane thickness under ultrasound, and expression of RANKL and M-CSF in joint synovium all increased in the model control group (P<0.05); compared with the model control group, The above observed indicators in the T-614 group, MTX group, and T-614 combined with MTX group were all reduced (P<0.05); compared with the T-614 group and MTX group, the above observed indicators in the T-614 combined with MTX group were all reduced (P<0.05). 0.05); there was no statistically significant difference in the above observation indicators between the T-614 group and the MTX group (P>0.05). Conclusion RANKL and M-CSF are involved in the occurrence and development of joint synovial lesions in CIA rats. T-614 and MTX may delay the progression of joint synovial lesions by reducing the production of cytokines RANKL and M-CSF. T-614 and MTX has certain synergistic effects.

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Wang Qianqian, Wang Meimei

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Polytetrafluoroethylene, Collagen, Heparin, Endothelial Cells, Cell Adhesion

Abstract

Using low-temperature plasma technology to activate polytetrafluoroethylene (PTFE), carboxyl groups are introduced on the surface, and then two bioactive molecules, collagen and heparin, are modified on the surface of the material through covalent bonds to construct a biological model that mimics the extracellular matrix (ECM). Activation interface. X-ray photoelectron spectroscopy (XPS) and contact angle results confirmed that the PTFE surface was successfully modified. Platelet and cell adhesion experiments show that the ECM-like microenvironment can significantly reduce the adhesion and activation of platelets on the material surface, and can effectively promote the adhesion and growth of endothelial cells on the material surface. Compared with the material before modification, its cell adhesion rate can be increased nearly 10 times, and the material has good blood compatibility.

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Xia Lin, Chen Jingxiao, Teng Liping, Chen Jinghua

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Root-Knot Nematode, Rna Interference, Cuticle Collagen, Virus-Mediated Gene Silencing

Abstract

In order to clarify the relationship between the nematode cuticle collagen gene (Col) and root-knot nematode diseases, specific primers were designed using the predicted Col from the nematode genome, and the Meloidogyne incognitacol gene MiCol of Meloidogyne incognita was cloned. The complete coding region of this gene is 903bp long and encodes 300 amino acids. The sequence identity of the cloned MiCol and the predicted MiCol gene is as high as 99.67%, and the identity of the amino acid sequence is as high as 100%. Using virus-mediated gene silencing technology, it was introduced into tomato plants and inoculated with Meloidogyne incognita. After 60 days, the number of root knots of tomato plants treated with the silencing vector pTV-MiColi was reduced by 49.4% and 49.2% respectively compared with the empty vector control and clear water control. , indicating that MiCol gene silencing significantly reduced the infection number of Meloidogyne incognita.

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Huang Yonghong, Mei Mei, Zhang Jixiang, Ma Jinhui, Mao Zhenchuan

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Single Domain Antibodies, Lidamycin, Fusion Proteins, Antibody Drugs, Type Iv Collagenase

Abstract

Type IV collagenase is closely related to tumor invasion, metastasis, and neovascularization. As a tumor-targeting carrier, single-domain antibodies have many advantages that traditional antibodies cannot match. Using type IV collagenase as a target, single-domain antibodies The antibody was used as the carrier, and the potent enediyne anti-tumor antibiotic lidamycin (LDM) was used as the “warhead”. A new method combining DNA recombination and molecular assembly was used to prepare the light chain of the anti-type IV collagenase monoclonal antibody 3G11. The genetically engineered enhanced fusion protein VL-LDP-AE of the variable region single domain antibody VL and LDM. MTT results show that VL-LDP-AE has a strong killing effect on tumor cells cultured in vitro, with an IC50 of HT-1080 cells and KB cells. The values are 8.55¡Á10-12 and 1.70¡Á10-11mol/L respectively. VL-LDP-AE can also inhibit the formation of new blood vessels in the allantoic membrane of chicken embryos and destroy human umbilical vein endothelial cells (HUVEC) to form endothelial tubules. Animals The experiment was conducted to observe the efficacy of transplanted liver cancer 22 (H22) in mice. The tumor was inoculated subcutaneously, and administration began 72 hours later (two intravenous injections on the 3rd and 10th days after inoculation). Comparisons were made with the maximum tolerated dose, VL -The tumor inhibition rate of LDP-AE0.375mg/kg was 89.5%, the tumor inhibition rate of LDM0.05mg/kg group was 69.9%, and the tumor inhibition rate of mitomycin (MMC) 1mg/kg group was 35%.VL – The molecular weight of LDP-AE is 25.4kD, which is much smaller than other forms of antibody drug molecules. As a small, highly efficient antibody drug, VL-LDP-AE has good application prospects in targeted tumor therapy. In addition, its unique preparation method It can also be used as a technical platform for the preparation of new anti-tumor antibody-targeted drugs.

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Miao Qingfang, Shang Boyang, Ouyang Zhigang, Liu Xiaoyun, Zhen Yongsu

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Bergamot, Wolfberry, Skin, Hair, Superoxide Dismutase

Abstract

Objective: To study the effects of bergamot and wolfberry extracts on skin and hair growth in mice. Methods: Apply the extracts of bergamot and wolfberry to the hairless skin site of mice, and observe the hair growth at the applied site. After 42 days, the skin from the applied site was taken to determine the superoxide dismutase SOD activity, collagen and malondialdehyde content. Results: Compared with the blank control group, both bergamot and wolfberry extracts could significantly increase the activity of SOD in mouse skin (P<0.05), increase the content of collagen in the skin (P<0.001), and significantly reduce lipid peroxidation products. The content of malondialdehyde (P<0.05) promotes hair growth (P<0.001). Conclusion: Bergamot and wolfberry extracts can nourish skin and promote hair growth. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Shao Linxiang  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Lumbar Disc Herniation, Laser, Collagenase

Abstract

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by He Jianrong, Wu Yongjun, Geng Qi, Qi Lianjun

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Lupus Erythematosus, Systemic, Collagen Lectin, Diagnosis

Abstract

Summary

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Liu Xiangyuan, Sun Lin, Li Changhong, Deng Xiaoli, Zhong Lijun

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Interlocking Intramedullary Needles, Fracture Healing, Dynamic Compressive Stress, Type I Collagen, Osteocalcin

Abstract

Objective To explore the effects of new dynamic compression interlocking intramedullary needles on the histology of fracture healing and the expression changes of type I collagen and osteocalcin in the callus. ? method? Fifteen 2-year-old green goats were used to create a bilateral mid-shaft transverse fracture model of the femur. The experimental side was fixed with a new type of dynamic compression interlocking intramedullary needles in order to form a sustained dynamic compressive stress condition at the fracture end. The control side was fixed with ordinary intramedullary needles. Pin fixed. On the 7th, 14th, 30th, 60th, and 90th day after surgery, samples were collected from the fracture healing site for histopathological and RT-PCR detection. Results Histological examination showed that the calcification of the callus matrix, endochondral osteogenesis and woven bone reconstruction in the fracture gap in the experimental group were faster than those in the control group. RT-PCR detection showed that the expressions of type I collagen and osteocalcin in both groups increased after fracture surgery, reaching peaks at 14 and 30 days after surgery respectively, and the experimental group was significantly higher than the control group (P<0.05). Conclusion The new dynamic compression interlocking intramedullary needle can promote the synthesis and secretion of osteoblast extracellular matrix and endochondral osteogenesis, thereby effectively accelerating the fracture healing process. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Luo Gang, Diao Xinwei, Li Changqing, Zhou Yue  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Bactrian Camel, Cryptorchidism, Laminin, Collagen, Heparan Sulfate Glycoprotein

Abstract

Abstract In order to explore the distribution and histochemical characteristics of extracellular matrix-related proteins in cryptorchid Bactrian camels, electron microscopy and various histochemical methods were used to compare the ultrastructure, histochemical characteristics and laminar adhesions of cryptorchid and normal testes. Distribution characteristics of protein (LN), type IV collagen (Col IV) and heparan sulfate glycoprotein (HSPG). The results show that: (1) Compared with the normal testicular interstitial structure, cryptorchid seminiferous tubules under light microscopy are underdeveloped, collagen fibers in the interstitium are sparse, reticular fibers are obviously distributed, interstitial blood vessels and the membrane proper of seminiferous tubules PAS and AB-PAS positive reaction is weak. Under the electron microscope, the basement membrane of cryptorchid spermatogenic epithelium is significantly proliferated, with fewer peripheral type I collagen fibers and atypical peritubular myoid cells; fibroblasts are more common around interstitial capillaries and Leydig cells, while in normal testes, interstitial capillaries are present. Fibroblasts are mostly distributed around Leydig cells. (2) Immunohistochemistry staining showed that Col IV, LN and HSPG in normal testicular tissue were all strongly positively expressed in Leydig cells, and Col IV and LN were strongly expressed in capillary endothelial cells, with the latter being especially expressed in Sertoli cells. Obviously, HSPG is not expressed in spermatogonia; in cryptorchidism, the positive expression of Col ¢ô, LN and HSPG in Leydig cells is significantly weakened, and the positive expression of Col ¢ô and LN in peritubular myoid cells and capillary endothelial cells is also significantly weakened. HSPG is strongly positively expressed in spermatogonia and sperm cells. Immunohistochemistry image analysis results showed that the distribution of Col IV and LN in the normal testicular tissue of Bactrian camels was significantly higher than that in cryptorchidism tissue (P<0.05). The HSPG test results showed no statistical difference between normal testicles and cryptorchidism (P >0.01). This study shows that the development of seminiferous tubules in Bactrian camel cryptorchidism is abnormal, the ability to synthesize collagen fibers in the interstitial tissue is reduced, and the important components of testicular extracellular matrix, Col IV and LN, are significantly different from the normal group and are related to abnormalities in seminiferous tubules and Leydig cells. It is related to development, and the strong positive expression of HSPG in cryptorchid spermatogenic epithelium is closely related to the immaturity of spermatogonia.

For further information of this article and research, feel free to contact our team for asssitance.

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

For further information of this article and research, feel free to contact our team for asssitance.

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.