Collagen Research

Keywords

Growth Hormone, Bare Process Chondrocytes, Proliferative Activity, Type Ii Collagen

Abstract

Objective To understand the effects of growth hormone on the cell cycle, proliferation activity and secretory function of the rabbit nudicollar cartilage cultured in vitro, and to provide a reliable experimental basis for the growth and reconstruction of the nudal process during functional orthopedic treatment. Methods Flow cytometry and immunohistochemistry methods were used to quantitatively and qualitatively observe the effects of different concentrations of growth hormone on rabbit subcollar bare process chondrocytes cultured in vitro at different time points. Results ¢Ù Different concentrations of growth hormone can stimulate the proliferation activity of chondrocytes in the lower collar nudity. The proliferation index (PI) changes are most significant in the growth hormone 10pg/ml, 24h group, and the DNA synthesis changes are most significant in the 10r-tg/ml, 12h group. The most obvious. ¢Ú Growth hormone has the ability to promote the secretion of n-type collagen by chondrocytes of rabbit inferior collarbone process, and the growth hormone 10% group is the most significant. Conclusion Growth hormone can promote the proliferation activity and the ability to secrete type II collagen of rabbit inferior collarbone chondrocytes cultured in vitro.

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Original research was done by Huang Ning, Luo Songjiao, Yang Hongmei

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Sinoatrial Node, P Cells, Cardiomyocytes, Collagenase, Rat

Abstract

A method for isolating and preliminarily identifying rat sinoatrial node pacemaker cells using collagenase is introduced. The operation is simple and convenient, and the amount of enzyme used is small. The isolated pacemaker cells have similar action potential characteristics to the in situ sinoatrial node pacemaker cells: there is obvious automatic depolarization during diastole, the average maximum diastolic potential is -55mV, the average action potential amplitude is 58mV, the average APD50 is 18ms, and the average APD90 29ms. Provide appropriate specimens for further electrophysiological and histochemical studies.

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Original research was done by Zeng Yingming, Li Che

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Collagen Fiber, Template, So2-4/Zro2 Solid Acid, Esterification Reaction

Abstract

SO2-4/ZrO2 solid acid was prepared using skin collagen fibers as template. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and ammonia temperature-programmed desorption were used to prepare the SO2-4/ZrO2 solid acid. (TPD), X-ray diffraction (XRD), specific surface area and pore size analysis, etc., characterized the structure and physical properties of SO2-4/ZrO2 solid acid. The results showed that SO2-4/ZrO2 relatively intactly maintained the collagen fibers. The fibrous structure. SO2-4 is coordinated and chelated on the surface of ZrO2 to form a solid super acid. As the heat treatment temperature increases, the specific surface area and average pore size of SO2-4/ZrO2 decrease, and its medium-strength acidity The center gradually weakens until it disappears. In the esterification reaction of citric acid, the SO2-4/ZrO2 solid acid with a fibrous structure shows high catalytic activity, and the catalyst has good reusability.

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Original research was done by Liao Yang, Huang Xin, Liao Xuepin, Shi Bi

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Ligustrum Lucidum Osteoporosis Calcium And Phosphorus Metabolism Collagen Metabolism Bone Structure

Abstract

Objective To explore the effect of Ligustrum lucidum water extract on bone protection and bone metabolism regulation in osteoporosis rats. Methods Ovariectomy was used to create a rat osteoporosis model. In the sham operation group, only the fat around the ovaries that was the same size as the ovaries was removed. The experiment was divided into 4 groups: sham operation group, model group, alendronate sodium (0.12 g/kg) group, and Ligustrum lucidum water extract (3.5 g/kg) group, with 9 animals in each group. Each group was administered ig for 12 consecutive weeks. At the end of the experiment, serum calcium (S-Ca), serum phosphorus (SP), urinary calcium (U-Ca/Cr), urinary phosphorus (UP/Cr) and serum high-density lipoprotein cholesterol (HDL-C) were measured using biochemical methods. ), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC) and triacylglycerol (TG); serum type I collagen amino-terminal propeptide (PINP) and type I collagen carboxyl-terminal peptide were measured using ELISA (CTX-I), osteocalcin (OCN) and urinary deoxypyridine (DPD); the activity of serum alkaline phosphatase (ALP) was measured by radioimmunoassay; evaluated by bone densitometer, VivaCT and universal testing machine respectively Structural changes in bone tissue. Results Ligustrum lucidum water extract can significantly inhibit the increase in body weight of rats caused by ovariectomy, increase the levels of S-Ca, SP, HDL-C and PINP, and reduce the levels of U-Ca/Cr and UP/Cr in ovariectomized rats. , reducing the levels of LDL-C, TC, TG, ALP, OCN and CTX-I in serum and the amount of DPD in urine (P<0.05, 0.01). Ligustrum lucidum water extract can increase the bone mineral density (BMD) of the femur and vertebrae in ovariectomized rats, improve the microstructure of the femur, and increase bone strength. Conclusion Ligustrum lucidum aqueous extract can increase BMD and bone strength in ovariectomized rats, and its mechanism of action is related to the regulation of calcium and phosphorus metabolism, collagen and non-collagen protein metabolism. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Haixia, Tang Yuqing, Zhang Dongwei, Zhu Ruyuan, Li, Yang Meijuan, Liu Chenh, Niu Jianzhao, Wang Lili, Wang Liang, Mo Fangfang, Zhao Dandan, Guo Yubo, Ma Rufeng, Gao Sihua  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Tang Lixia Yang Guang Tan Jiaju

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

? , Skin Jelly, Soup Dumplings, Meat Skin, Collagen, Formation Mechanism, Boiling, Analysis, Filling, Gel, Polypeptide Chain

Abstract

The formation mechanism of soup dumpling skin jelly was discussed. Analyzing the main changes and characteristics of the formation of skin jelly from the production process, it is explained that the formation of skin jelly mainly goes through the two processes of collagen denaturation and network structure formation, and that the change between the solid and liquid states of skin jelly is just one substance. changes in attitude.

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Original research was done by Mao Yuyang

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Integrin ¦¡2¦¢1, Hepatocellular Carcinoma Cells, Type Iv Collagen, Adhesion, Chemotaxis, Micropipette Technology

Abstract

Micropipette technology was used to study the effect of integrin ¦Á2¦Â1 on the adhesion characteristics of hepatocellular carcinoma (HCC) cells SMMC-7721 and type IV collagen lining surfaces and the chemotactic behavior of type IV collagen of the cells. Observe the effects of Anti-¦Á2 and Anti-¦Â1 on the adhesion of SMMC-7721 cells to type IV collagen-coated surfaces; use the double micropipette experiment method to observe the pseudopod formation process of SMMC-7721 cells and the effects of Anti-¦Á2, Anti- Effect of ¦Â1 on pseudopod formation in SMMC-7721 cells. Flow cytometry was used to analyze the expression of integrin ¦Á2 and ¦Â1 subunits on the surface of SMMC-7721 cells. The results showed that the adhesion force between SMMC-7721 cells and the surface lined with 5 ¦Ìg/mL type IV collagen was 952¡À134 (¡Á10^-10N, n=60). The adhesion force decreased to 605¡À128 (¡Á10^-10N, n=60); when adding 10¦Ìg/mL Anti-¦Á2, the adhesion force decreased to 579¡À57 (¡Á10^-10N, n=50). The adhesion force decreased to 449¡À119 (¡Á10^-10N, n=60) when 5 ¦Ìg/mL Anti-¦Â1 was added; the adhesion force decreased to 220¡À78 (¡Á10^-10N, n=60) when 10 ¦Ìg/mL Anti-¦Â1 was added. n=55). The double micropipette chemotaxis experiment showed that when the same concentration of type IV collagen was added to both sides of the micropipette, pseudopodia were formed in the cells on both sides of the micropipette. On this basis, Anti-¦Á2 and Anti-¦Â1 were added to one side of the micropipette respectively. , the pseudopod growth curve of SMMC-7721 cells showed obvious inhibition, and compared with the opposite side where the antibody was not added to the side of the micropipette, the pseudopodia of the cells on this side increased significantly. Flow cytometry analysis showed that the expression rates of integrin ¦Á2 and ¦Â1 subunits in the cells studied were 23.17% and 95.78% respectively. The above results indicate that integrin ¦Á2¦Â1 is an important receptor basis for the adhesion of SMMC-7721 liver cancer cells to type IV collagen-coated surfaces and the formation of chemotactic pseudopodia of SMMC-7721 cells for type IV collagen.

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Original research was done by Fu Panhong, Zhang Shulin, Wu Zezhi

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Seasoning, Animal Blood, Proline, Decomposition, Glycine, Leucine, Neutralization Filtrate, Exchange Protein, Collagen, Boiled Water

Abstract

? Collagen from animal hides and tendons is used to make seasonings after decomposition, which contains a lot of proline, oxidized proline, glycine, etc., but a small amount of essential amino acids, because hemoglobin contains essential amino acids.

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Original research was done by Fang Zilai

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Red Stingray, Angiogenesis Inhibitory Factor, Purified, Collagenase, Chick Chorioallantoic Membrane

Abstract

The purification process for preparing high-purity red stingray cartilage angiogenesis inhibitory factor was determined, and its biological activity was verified through collagenase model and CAM model. Orthogonal experiments determined the optimal conditions for extracting red stingray cartilage tissue, and the extraction yield could reach 0.84%. Acetone fractionated precipitation was used to roughly separate the extraction products, and the CAM angiogenesis inhibition rate of 25-35% acetone fractionated products reached 85%. By optimizing the conditions of ion exchange chromatography, gel filtration, and reversed-phase chromatography, high-purity red stingray cartilage angiogenesis inhibitory factor-I (DCAIF-I) can be further effectively obtained. SDS-PAGE-Coomassie brilliant blue staining showed a band with a molecular weight of 62kDa. Activity verification results show that DCAIF-I has a certain inhibitory activity on collagenase, with an inhibitory rate of 36.3%, and its inhibitory activity on CAM angiogenesis has a certain dose-dependent relationship.

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Original research was done by Yu Xinwei, Qian Xiao, Wang Jieyu, Luo Hongyu, Wu Changwen

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Pig Skin, Enzymatic Hydrolyzate, Emulsifying Activity, Emulsifying Stability

Abstract

Three types of porcine skin collagen enzymatic hydrolysates (PCH 1, PCH 2, and PCH 3) with different molecular weight segments were separated by ultrafiltration, and the characteristics of the three enzymatic hydrolysates at different pH values, soluble protein concentrations, and Emulsification ability and emulsification stability under three conditions of oil volume fraction. The results show that pH value, soluble protein concentration and oil volume fraction can significantly affect the emulsification ability and emulsification stability of PCH 1, PCH 2 and PCH 3 (P<0.05), and the soluble protein concentration has an important effect on the emulsification ability. has the greatest impact. The emulsifying abilities of PCH 2 and PCH 3 were significantly greater than PCH 1 (P<0.05). Molecular mass analysis results show that polypeptide solutions with smaller molecular masses have better emulsifying properties. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Yu Donghua, Zhou Lingling, Zhang Yehui, Xu Zhihong, Wei Zhencheng, Wang Chong  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.