Collagen Research

Keywords

Oligopeptides, Molecular Weight Distribution, Determination Methods

Abstract

Objective To establish a method for determining the relative molecular weight distribution of small molecule oligopeptides in fish skin collagen hydrolyzate. Method: High-performance gel exclusion chromatography was used with three TSK-GEL G2000SWXL columns (300 mm¡Á7.8 mm, 5 ¦Ìm) connected in series at room temperature; the mobile phase was acetonitrile-0.1% trifluoroacetic acid solution (15:85), flow rate 0.6 mL?min-1, detection wavelength 220 nm. Results: Within the relative molecular weight range of 149 to 2 490 Da, the logarithm of the relative molecular weight of the oligopeptide had a good linear relationship with its retention time (r=0.995 6); both precision and repeatability RSD were <0.1%. Conclusion This method is simple, accurate and reproducible, and can be used as a method to determine the molecular weight distribution of small oligopeptides. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Ye Xiaodi, Zhou Yangang, Cheng Min, Miao Yunping, Jia Jianping, Zheng Gaoli, Chen Aiying  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Simvastatin, Arthritis, Anti-Inflammatory Effect, Tumor Necrosis Factor Alpha, Interleukin 6, Caspase-3

Abstract

Objective To use collagen-induced arthritis (cia) rats as a model to observe the effect of simvastatin on the expression of serum cytokines and synovial tissue aspartate proteolytic enzyme-3 (caspase-3), and explore the treatment of simvastatin Possible mechanisms of rheumatoid arthritis. Methods Bovine type ¢¢ collagen was used to establish the cia model. The rats (16 rats) that were successfully modeled were equally divided into the model group and the drug group. The rats that were not modeled (7 rats) were the control group. The drug group was given 2.0 mg/(kg¡¤d) by gavage, and the control and model groups were given 5 ml/(kg¡¤d) sterile water for injection. The arthritis score (ai) and toe volume of the rats were recorded once a week; on the 42nd day after the initial immunization, blood was taken from the inner canthus of the eyes and the ELISA method was used to detect serum tumor necrosis factor (tnf)-¦Á and interleukin in each group. (il)-6 level, the rats were sacrificed, and the hindlimb ankle joints were harvested for HE staining, and immunohistochemistry was used to detect the expression of caspase-3 in synovial tissue. Results The ai of the drug group and the model group began to decrease 21 days after the initial immunization, and after 35 days, the drug group was lower than the model group (all p<0.05). The toe volume of the two groups decreased slowly 14 days after the initial immunization, but was still significantly higher than that of the control group; the drug group was lower than the model group on 35 and 42 days (both p<0.05). 42 days after the initial immunization, the levels of tnf-¦Á and il-6 in the drug group were lower than those in the model group and higher than those in the control group (all p<0.05). Compared with the model group, the drug group showed significantly reduced ankle synovial tissue hyperplasia, with only a small amount of inflammatory cell infiltration, and the expression of caspase-3 was significantly increased. Conclusion Simvastatin can significantly inhibit the secretion of serum tnf-¦Á and il-6 in cia rats, upregulate the expression of caspase-3 in synovial tissue and reduce synovial tissue hyperplasia. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Wang Youlian, Yang Mingfeng, Shang Ke, Pi Hui  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by Zhang Linsheng, Lu Ping, Cao Rang

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Inorganic Ceramic Materials, Surface Modification, Silane Coupling Agent, Silicon Carbide, Dispersion, Flexural Strength

Abstract

Silane coupling agent KH550 was used as surfactant to modify the surface of silicon carbide powder. Scanning electron microscope, X-ray diffractometer, laser particle size analyzer and infrared spectrometer were used to study the morphology, surface properties and Particle size distribution and micromorphology of sintered body. The results show that the silane coupling agent and silicon carbide form a coating layer on its surface through a grafting reaction, but its phase and structure are not changed. After modification, the agglomeration of silicon carbide powder is reduced, so the average particle size is reduced; the electrostatic repulsion and steric hindrance between particles are increased, which improves the suspension stability and dispersion of silicon carbide particles in the sol. Compared with unmodified SiC, the sintered body of SiC and ceramic modified with sol-gel in-situ molding SiC/ceramic composite has a uniform structure and higher flexural strength

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Original research was done by 10.11901/1005.3093.2016.494

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

Titanium was loaded on collagen fibers to prepare an adsorbent material (TICF), and the adsorption law of UO2+2 in simulated uranium fluoride wastewater by this adsorbent material was systematically studied. The results show that TICF has strong adsorption capacity for UO2+2. When the temperature is 303K, the pH is 5.0, and the initial concentration of UO2+2 is 1.50mmol¡¤L-1ªª, the adsorption capacity reaches 0.62mmolU¡¤g-1ªª ; TICF service recommends this article to friends (¢ô); Adsorption; Uranium fluoride wastewater” several good articles, specially recommended to you. Please click the following URL: “name=”neirong”>Join the citation manager E-mailAlertRSS Author related article

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Original research was done by Cheng Yuanmei, Sun Xia, Liao Xuepin, Zeng Fanjun, Wu Yun, Shi Bi

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Bacillus Anthracis, Bcla Protein, Polymorphism, Spores

Abstract

Bacillus anthracis spore exine wall collagen-like protein (BclA) is the main structural component of the hairy hyphae on the spore exine wall and is also the main immunogen of the spore. Bc lA genes were cloned from three strains of Bacillus anthracis isolated in China and sequence analyzed. It was found that the length of Bc lA of two strains (A16R and 40048) was different from that of foreign reported strains, containing 388 and 322 amino acids respectively, and 72 There are 5 and 50 GXX three-amino acid repeats, and 5 and 3 5GDTGTT repeats (GPT) containing 21 amino acids (BclA repeats). The Bc lA of another strain 40022 is exactly the same as that of the 53169 strain reported abroad, containing 370 amino acids, 66 GXX repeats, and 5 Bc lA repeats. to our country

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Original research was done by Li Guanlin, Xu Junjie, Dong Dayong, Song Xiaohong, Chen Wei

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

In China, the specific repeat sequence of the Y chromosome has been used as a probe for DNA hybridization to identify gender in early pregnancy and has been successful. The Genetic Research Laboratory of Shanghai Children’s Hospital has further improved and established a DNA gel in situ hybridization method for gender identification. This article applies this method to study patients with abnormal sexual differentiation. Materials and methods The specimens were collected from patients in endocrinology clinics, a total of 2 cases. A case of simple XY gonadal dysplasia

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Original research was done by Yu Chuanxin, Lu Juxiang, Huang Ying, Zhang Meilan, Xue Jinrong, Zeng Yitao

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Keywords: Fragility Fracture, Hip, Elderly, Type ¢ñ Procollagen Amino-Terminal Extended Peptide, Type ¢ñ Collagen Carboxyl-Terminal Peptide Beta Special Sequence, 25-Hydroxyvitamin D3

Abstract

Abstract: Objective To explore the effects of serum total bone type I procollagen amino-terminal extension peptide (total-p1np), type I collagen carboxyl telopeptide beta special sequence (¦Â-ctx), and 25(oh)d3 in elderly people with hip fragility fractures. the value of. Methods 68 elderly osteoporosis patients with a history of hip fragility fracture were collected. At the same time, 68 age- and gender-matched elderly patients with confirmed osteoporosis and no history of hip fragility fracture were set up as controls. The serum total- p1np, ¦Â-ctx, 25(oh)d3; dual-energy x-ray absorption method was used to determine lumbar spine and hip bone mineral density (bmd); spss16.0 statistical software was used for statistical analysis. Results (1) The total-p1np and ¦Â-ctx of patients in the hip fragility fracture group were higher than those of the control group, and the 25 (oh) d3 was lower than that of the control group. The difference between the groups was statistically significant (p<0.05); the lumbar spine between the two groups There was no statistical difference between mean bmd and total hip bmd; (2) Bivariate correlation analysis found that in the hip fragility fracture group, 25 (oh) d3 was positively correlated with lumbar mean bmd and total hip bmd, total-p1np, ¦Â-ctx was negatively correlated with lumbar spine mean bmd and total hip bmd (p<0.05); in the control group, 25 (oh) d3 had no correlation with lumbar spine mean bmd and total hip bmd, total-p1np, ¦Â-ctx and There was no correlation between the mean bmd of the lumbar spine and a negative correlation with the total hip bmd (p<0.05). Conclusion In elderly osteoporosis patients with similar bmd levels, increases in total-p1np and ¦Â-ctx and decreases in 25(oh)d3 may independently predict an increased risk of hip fragility fractures. The detection of these three indicators is It is helpful to identify people at high risk of hip fragility fracture and guide treatment. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Lou Huiling, Peng Cheng, Chen Qiaocong  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Bionic Spinal Cord Scaffold, 3-D Printing, Neural Stem Cells, Biocompatibility

Abstract

Objective To use 3-D bioprinter to prepare collagen-heparin sulfate bionic spinal cord scaffold to provide cell carrier for tissue engineering treatment of spinal cord injury. Methods Heparin sulfate-collagen hydrogel was prepared, and a 3-D printed bionic spinal cord scaffold was used. Its structure was observed with a scanning electron microscope, the porosity was calculated by the drainage method, and the pH value and quality changes of the scaffold were measured by an in vitro degradation experiment. Neural stem cells (NSCs) were isolated and cultured from the fetal cerebral cortex of SD rats on the 14th day of pregnancy. The experiment was divided into two groups. Group A used bionic spinal cord scaffolds to co-culture with NSCs in vitro, and group B directly inoculated the cell suspension. on 24-well culture plates precoated with L-polylysine. Light microscopy and scanning electron microscopy were used to observe cell adhesion and morphological changes, MTT was used to detect cell viability, and immunofluorescence staining was used to identify the differentiation of NSCs. Results The 3-D printer successfully prepared a collagen-heparin sulfate bionic spinal cord scaffold. Scanning electron microscopy showed that the interior had a longitudinally arranged and parallel microporous structure with a porosity of 90.25% ¡À 2.15%. In the in vitro degradation experiment, the pH value of the scaffold did not change. Significant changes occurred, and the scaffold was completely degraded around 8 weeks, meeting the requirements of tissue engineering scaffolds. MTT assay showed that there was no statistically significant difference in the absorbance (A) values between the two groups at 1, 3, and 7 days of culture (P>0.05); light microscopy observation showed that a large number of neurospheres were differentiated in both groups, and nerve fibers were interwoven into a network; cultured 7 d Scanning electron microscopy observation of group A showed that cells adhered to the scaffold, a large number of cells extended axons, and neurospheres were formed. Immunofluorescence staining showed that both groups could differentiate into neurons and glial cells. Quantitative analysis showed that A. The differentiation rates of group B were 29.60%¡À2.68% and 10.90%¡À2.13% respectively, and the difference was statistically significant (t=17.30, P=0.01). Conclusion The 3-D printed collagen-heparin sulfate bionic spinal cord scaffold has good biocompatibility, can promote the proliferation and differentiation of NSCs, and has good research and application prospects as a neural tissue engineering scaffold.

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Original research was done by Zhang Sai, Li Xiaohong, Liang Haiqian, Tu Yue, Wang Jingjing, Zhao Mingliang, Chen Chong

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

? ,Collagenase,Trypsin,Osteoblast,Cell Culture,? , Collagenase, Trypsin, Osteoblasts, Cell Culture

Abstract

Objective: To explore the best method for establishing in vitro culture of neonatal mouse osteoblasts and to provide a stable and reliable source of seed cells for bone tissue engineering research. Methods: The skulls of neonatal mice (within 48 hours) were used to culture cells in vitro using a modified two-step digestion method of trypsin and collagenase. The cell morphology was observed under an inverted microscope, and the alkaline phosphatase (ALP) activity and mineralization ability were observed. Identify and draw cell growth curves to observe the phenotypic characteristics of osteoblasts. Results: Primary cells inoculated 24? After 1 hour, some round cells can be seen adhering to the wall in a scattered manner. At 48 to 72 hours, they completely adhere to the wall and stretch into a plump and polygonal shape, forming multiple colonies. At 7 to 8 days, the number of colonies increases rapidly. By about 10 to 15 days, the cells enter the rapid growth phase. The cells in the center of the colony are densely packed, and the surrounding cells are arranged in a radial or swirling pattern. There is no contact inhibition between cells, and there is overlap between colonies; the fused cells grow in overlapping and stratified layers after 15 to 20 days. The morphology of cultured osteoblasts shows irregular polygons, mostly irregular triangles or quadrilaterals, with short and thin protrusions on the surface. Hematoxylin staining, alkaline phosphatase staining and alizarin red staining of mineralized nodules confirmed that they had a complete osteoblastic phenotype and were indeed osteoblasts. Conclusion: The improved collagenase and trypsin combined digestion method can obtain high-purity osteoblasts in a short time, and is expected to become a routine method for seed cell culture in bone tissue engineering. ª£

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Original research was done by Na Jian, Ma Chao, Huo Weiling, Qin Ruiyun, Xu Yong, Wang Tao, Wu Xiaodong, Gu Guishan

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.