Collagen Research

Keywords

Abstract

Abstract In order to reveal the effects of different extraction methods on the structural characteristics and functional properties of plaice skin collagen, fresh plaice skin was used as the research object, and collagen was extracted from it using acid method and enzymatic method to obtain acid-soluble collagen (ASC) and Enzymatically soluble collagen (PSC) was studied, and the structural characteristics and functional properties of these two collagens were comparatively studied. The UV spectrum scanning results showed that both ASC and PSC had strong absorption peaks at 230 nm and no peaks at 280 nm. The obvious absorption peaks are consistent with the structural characteristics of collagen; Fourier transform infrared spectrum scanning results show that both ASC and PSC contain amide A, amide B, amide I, amide II, and amide III, and both maintain the three-dimensional helical structure of collagen. Integrity; SDS-PAGE gel electrophoresis confirmed that both ASC and PSC are type I collagen. The results of hygroscopicity and moisturizing properties showed that with the change of time, the hygroscopicity rates of ASC and PSC showed a gradually increasing trend, while their The moisture retention rate showed a downward trend; the results of turbidity and aggregation characteristics showed that the aggregation kinetic curves of ASC and PSC solutions both showed an S-shaped trend, and the aggregation capacity of ASC was greater than that of PSC during the entire process; water absorption and oil absorption The results show that both ASC and PSC have certain water and oil absorption properties, and the oil absorption properties of both are greater than their respective water absorption properties. The above results show that different extraction methods have certain effects on the structural characteristics and functional properties of sole fish skin collagen. Influence

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ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Konjac Glucomannan Microspheres, Gelatin, Conjugation, Microcarriers, Cell Culture

Abstract

Konjac glucomannan (kgm) microspheres are used as the matrix, the kgm microspheres are activated with 1,4-butanediol diglycidyl ether, collagen is coated on the microspheres, and the collagen coating is cross-linked again , to obtain microcarriers with uniform and stable coating. Through the orthogonal regression combination experimental design of four factors and three levels, the effects of activation time, protein dosage, coupling time, and cross-linking agent dosage on the microcarrier cell culture effects were investigated. Taking the vero cell culture effect as an indicator, the optimal process for preparing collagen-coated microcarriers is activation time 5h, protein dosage 1:0.1 (ball:protein), coupling time 5h, and cross-linking agent dosage of 0.5ml per 1gkgm. agent. Under optimal preparation conditions, the maximum cell density of cultured vero cells can reach 1.7¡Á106 cells/ml, proving the feasibility of collagen-coated kgm microspheres as microcarriers for animal cell culture.

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Original research was done by Kang Jiyao, Zhang Ning, Zhou Weiqing, Sun Lijing, Zhang Guifeng, Ma Guanghui, Su Zhiguo

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Collagen Type ¢ñ Fibrillar Collagens Hydroxyapatites Biocompatible Materials Rats Tail

Abstract

Purpose: Use rat tail type I collagen to construct bionic bone materials that are similar in chemical composition and molecular structure to human autologous bone tissue. Methods: Rat tail type I collagen was extracted through acetic acid hydrolysis and reconstructed into collagen fibers. Then, the reconstructed collagen fibers were placed in the mineralization solution to simulate bone biomineralization for 2 to 6 days, and the bone biomineralization was observed through transmission electron microscopy and electron diffraction. Results: The rat tail type I collagen extracted by acid hydrolysis can be reconstructed into collagen fibers and has a characteristic D-Band structure. Transmission electron microscopy and electron diffraction showed that: after 2 days of mineralization, the calcium hydroxyapatite precursor penetrated into the collagen fibers, and the collagen fibers were partially mineralized; after 6 days of mineralization, the interior of the collagen fibers was completely mineralized, forming type I collagen/ Biomimetic bone material based on calcium hydroxyapatite. Conclusion: Rat tail type I collagen extracted by acid hydrolysis can be reconstructed into collagen fibers, and after mineralization can form bionic bone materials consistent with the chemical composition and molecular structure of human autologous bone tissue. Abstract: Objective: To produce bionic bone material that is consistent with human bone in chemical composition and molecular structure using rat tail tendon collagen type ¢ñ. Methods: The type ¢ñcollagen derived from rat tail was extracted by acetic acid to form collagen fibers. The reconstructed collagen fibers were placed in the mineralized solution to mimic bone mineralization for 2-6 days. Bone mineralization was observed by transmission electron microscopy and electron diffraction.Results: Collagen fibers with characteristic D-Band structure were reconstructed by using rat tail tendon collagen type ¢ñ extracted with acid hydrolysis method. Transmission electron microscopy and electron diffraction showed that calcium hydroxyapatite precursor infiltrated into the collagen fibers, and the collagen fibers were partially mineralized after 2 days of mineralization; the collagen fibers were completely mineralized and bionic bone material of type¢ñ collagen/ calcium hydroxyapatite was formed after 6 days of mineralization.Conclusion: The collagen type I can be extracted from rat tail tendon by acid hydrolysis method, and can be reformed and mineralized to form the bionic bone material which mimics human bone in chemical composition and the molecular structure. Key words: Collagen type ¢ñ Fibrillar collagens Hydroxyapatites Biocompatible materials Rats Tail

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Original research was done by Zhang Zhan, Zhang Chun, Guo Qiaofeng

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Pulmonary Function, Tuberculosis, Lung, Serum Fibrosis Index, Type Iv Collagen, Type Iii Procollagen

Abstract

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Original research was done by Ge Chuan, Chen Yulong

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Zhuanggu Shenjin Capsule Osteoporosis Type I Collagen Bone Morphology

Abstract

Objective: To study the therapeutic effect of Zhuanggu Shenjin capsule (ZGSJC) on ovariectomized osteoporosis rats, and to explore the mechanism of ZGSJC in treating postmenopausal osteoporosis. Methods: Ovariectomized rats were immediately divided into model group, positive drug combined with estrogen group, ZGSJC high, medium and low dose group (crude drug 5.42, 2.71, 1.36 g¡¤kg-1), and a sham operation group. Each group 12. At the end of the experiment, non-decalcified bone sections were taken from the right tibia for bone morphometry examination. The protein from the left tibia was extracted and the expression of type ¢ñ collagen was determined by Western blotting. Results: Compared with sham surgery, the trabecular bone volume percentage (TBV), trabecular bone resorption surface percentage (TRS), trabecular bone formation surface percentage (TFS), trabecular bone formation surface percentage, and trabecular bone volume percentage of rats in the model group were significantly reduced (P<0.01). The mineralization rate (MAR), osteoid average width (OSW), and cortical mineralization rate (mAR) were significantly increased (P<0.01), and the expression of type I collagen was significantly decreased (P<0.01); compared with the model group, ZGSJC can significantly inhibit the decrease of TBV% (P<0.01), inhibit the increase of TRS, TFS, MAR, and mAR (P<0.01, P<0.05), and increase the expression of type I collagen (P<0.01). For OSW, Obvious effect. Conclusion: Zhuanggu Shenjin Capsule has a certain therapeutic effect on osteoporosis in rats caused by ovariectomy, which is specifically reflected in regulating the high turnover state of bone metabolism caused by ovariectomy and increasing the expression content of type ¢ñ collagen. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Zhou Yawei, Sun Xiaoli, Li Lijuan, Wang Wei, Su Haifeng, Guo Qing  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Shrna Fibroblast Activation Protein Lung Tumor Microvessel Density Collagen

Abstract

Abstract: [Objective] To study the effect of shRNA interfering with the expression of fibroblast activation protein (FAP) on the growth of Lewis lung cancer in mice and explore the possible mechanism. [Method] C57 female mice were established with subcutaneous transplantation tumor and lung metastasis models, and were randomly divided into 3 groups: FAP-shRNA group, HK group, and 5% glucose (5% GS) group, which received FAP-shRNA interference plasmid liposome complex respectively. Treatment with substance, empty plasmid liposome complex and 5% glucose water. The size and weight of the transplanted tumors, the number of lung metastases and the wet weight of the lungs were recorded. The FAP expression level, tumor microvessel density, and collagen fiber status in each group were detected. [Results] The growth of subcutaneous transplanted tumors in mice in the FAP-shRNA group was slower than that in the 5% GS group and HK group. The number of lung metastasis nodules and lung wet weight in the FAP-shRNA group were both smaller than those in the 5% GS group and HK group. In addition, in the FAP-shRNA group, the expression of FAP was down-regulated, the density of tumor microvessels was reduced, the expression of type I collagen was increased, and the deposition of collagen fibers was increased and their distribution was disordered. [Conclusion] The anti-tumor effect of FAP-shRNA interference plasmid may be achieved by reducing tumor angiogenesis, increasing type I collagen expression, and ultimately changing the tumor microenvironment.

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Original research was done by 10.11735/j.issn.1671-170X.2014.10.B001

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Keywords Redox Factor 1, Fibroblasts, Cell Proliferation, Collagen

Abstract

Objective: To study the effect of redox factor 1 (ref1) on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts and its possible mechanism. Methods: In order to confirm the role of ref1 in the proliferation and collagen synthesis of neonatal mouse cardiac fibroblasts, recombinant ref1 wild-type and mutant adenoviral vectors were constructed to infect neonatal mouse cardiac fibroblasts. Changes in the expression of ref1 and genes related to collagen synthesis were detected by RT-PCR and western blotting, ref1 nuclear translocation was detected by immunofluorescence, cell proliferation and cell cycle were analyzed by MTT and flow cytometry, and electrophoretic mobility shift assay was used , EMSA) to detect the effect of ref1 on the DNA binding ability of the transcription factor activator protein 1 (AP1). A medium containing 25 mmol/l glucose was used to simulate a high-glucose environment, and the effects on the proliferation and ref1 expression and activity of neonatal mouse cardiac fibroblasts were observed. Results: MTT colorimetry showed that the optical density value at 490nm of cells infected with wild-type ref1 recombinant adenovirus was significantly higher than that of the control virus group (0.671¡À0.044vs0.364¡À0.007, n=6, p<0.01). Analysis of the cell cycle by flow cytometry showed that wild-type ref1 could significantly increase the percentage of S-phase cells (16.8%¡À0.62% vs 9.04%¡À0.43%, n=3, p<0.05). RTª²PCR detection found that wild-type ref1 promoted the increased expression of collagen type I (collageni, col¢¡) and collagen type ¢£ (collagen ¢£, col¢£) mRNA in cells, but mutant ref1 had no obvious effect on cell proliferation and collagen synthesis. EMSA experimental results show that cells infected with wild-type ref1 recombinant adenovirus have enhanced DNA binding ability of ap1. Culture of neonatal mouse cardiac fibroblasts in high-glucose medium can also enhance the DNA-binding ability of ap1. Although high-glucose culture had no effect on the expression level of ref1, it enhanced the nuclear translocation of ref1 in cells and promoted cell proliferation. Conclusion: ref1 promotes the proliferation and collagen synthesis of neonatal mouse cardiac fibroblasts, and the mechanism may be by upregulating the DNA binding ability of AP1. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Geng Yan, Li Tao, Hu Xiaoqing, Zhang Chenguang, Chen Ping, Ma Kangtao, Zhou Chunyan  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Type ¢ñ Collagen, P38 Mapk, Type ¢ó Collagen, Human Aortic Smooth Muscle Cells, Type ¢ó Collagen, Human Aortic Smooth Muscle Cells, P38 Mapk, Type ¢ñ Collagen

Abstract

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Original research was done by Wang Xiaolin, Zhou Yuanli, Sun Wei, Li LiWANG Xiaolin, ZHOU Yuanli, SUN Wei, LI Li

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Keywords: Chinonol, Bovine Type Ii Collagen, Lymphocytes, Rheumatoid Arthritis

Abstract

Abstract: Objective To observe the immunomodulatory effect of chinonol on bovine type II collagen-specific lymphocytes in vivo and in vitro, and to explore the immunosuppressive mechanism of chinonol on antigen-specific lymphocytes. Methods Wistar rats were randomly divided into control group, model group, chinonol group and prednisolone group. Except for the control group, the collagen-induced arthritis rat model was replicated and administration started on the day of modeling. After 30 days of modeling, the spleen and draining lymph nodes were removed, and single cell suspensions were prepared. After 48 hours of in vitro culture, interferon-¦Ã (ifn-¦Ã), interleukin-10 (il-10), and il- in the culture supernatant were detected by ELISA. 4. Tumor necrosis factor-¦Á (tnf-¦Á) content; under the induction of type II collagen, normal rat lymph node cells and spleen cells were co-cultured with different concentrations of chinonol to detect collagen-induced lymphocyte proliferation and apoptosis. . Results Compared with the model group, the contents of ifn-¦Ã and tnf-¦Á in the splenocyte culture supernatant of the chinonol group were reduced (p<0.05, p<0.01), and the levels of il-10 and il-4 were increased (p<0.05 , p<0.01); the contents of ifn-¦Ã and tnf-¦Á in lymph node cell culture supernatant decreased (p<0.05, p<0.001), and the levels of il-10 and il-4 increased (p<0.05, p<0.001) ; In in vitro experiments, chinonol inhibited the proliferation of type II collagen-specific lymph node cells and promoted the apoptosis of type II collagen-specific lymph node cells and splenocytes in a dose-dependent manner. Conclusion Chinonol can inhibit the secretion of pro-inflammatory factors by type II collagen-specific lymphocytes, promote the secretion of anti-inflammatory factors by collagen-specific lymphocytes, inhibit the proliferation and promote apoptosis of collagen-specific lymphocytes, and exert immunosuppression in multiple ways. effect. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Lu Yue, Xiao Juan, Wu Zaiwang, Wang Ming, Fu Hongzheng, Chen Yingyu, Qian Ruiqin  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Slow-Release Collagen Binding Domain Interleukin 4 Collagen I Slow-Release Collagen Binding Domain Interleukin 4 Collagen I

Abstract

Objective To recombinantly express the anti-inflammatory factor interleukin 4 (IL-4) that can be sustained-released. Methods Based on the amino acid sequence of mouse IL-4 and the gene for synthesizing IL-4 based on the codon preference of Escherichia coli, the collagen binding domain (CBD) was encoded by the polymerase chain reaction (PCR) method. The sequence and histidine tag sequence were connected to the IL-4 gene, and the above recombinant gene was inserted into the pET-30a(+) vector using seamless cloning technology. The recombinant protein was purified through histidine tags. The purified protein was used to induce the differentiation of M0 macrophages into M2 macrophages, while detecting the expression of genes related to M2 macrophages and the secretion of interleukin 10 (IL10). . Combined with type I collagen to verify the adsorption and sustained release functions of collagen to recombinant factors. Results IL-4 with CBD sequence and histidine purification tag was purified through nickel column and had similar biological activity to commercial IL-4. Functional experiments have confirmed that it can be adsorbed by collagen and can be released slowly from collagen. Conclusion The slow-release anti-inflammatory factor interleukin4(IL-4) gene was obtained through expression and purification in Escherichia coli. Objective To construct and identify the slow-release anti-inflammatory factor interleukin4(IL-4) gene. Methods The mouse IL-4 gene was synthesized according to its amino acid sequence(ACCESSION: NP_067258) and the E.coli codon usage preference. Collagen binding domain(CBD) and His Tag sequence were linked with IL-4 sequence by PCR amplification with primers containing CBD and His Tag sequence. PCR products were reassembled into the cloning vector pET-30a(+) by seamless cloning technique. After NI-sepharose purification, the bioactivity of fusion protein was tested by polarizing M0 macrophage to M2 macrophage through measuring the gene expression and interleukin10(IL10) secretion. In combination with collagen I, the adsorption and release of recombinant proteins by collagen I was tested. Results IL-4-CBD-His Tag was purified successfully by Nickel column and it had the similar bioactivity with commercial IL-4. The function assay indicated that IL-4-CBD-His Tag was able to bind to collagen I and to be slowly released from collagen. Conclusion The slow-release anti-inflammatory factor IL-4 gene has been successfully constructed through E.coli expression system

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Original research was done by Zhang Chong, Huang Zike, Li Siguang

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.