Collagen Research

Keywords

Collagen, Bone Morphogenetic Protein-2, Bmp-2, Bone Defect, Ectopic Bone Formation Collagen, Bone Morphogenetic Protein-2, Ectopic Osteogenesis, Bone Defect

Abstract

Collagen/bone morphogenetic protein composites were prepared with collagen type I sponge and bone morphogenetic protein-2 (BMP-2). The composites were implanted into Latissimus dorsi muscles pouches of rabbits. Samples were studied with ALP staining, Von Kossa staining, HE staining , toluidine blue staining and CD31 histochemical labeling of microvessel. Bony samples were then used to repair mandibular defect. The effects were evaluated by X-ray, compressive strength, economycin fluorescence labeling, HE staining, toluidine blue staining and bone quantity analysis. Bone formation induced by collagen/BMP composites was found as woven bone between 4 and 6 weeks; cartilaginous osteogenesis was the main type of bone formation; microvessels could be seen in the bony tissues; and the bone defects were healed completely 6 weeks after operation. Bone formation induced by collagen/BMP composites in the muscles can be used as a donor to repair the bone defect.

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Original research was done by Weiqiang Tan,Jinghong Xu,Youshan Liu,Jun Lin,Huiming Wang, Tan Weiqiang, Xu Jinghong, Liu Youshan, Lin Jun, Wang Huiming

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Tuna, Fish Bone, Peptides, Antioxidant Activity

Abstract

In order to prepare tuna fish bone collagen peptide and study its antioxidant activity, enzymatic hydrolysis, ultrafiltration, gel chromatography and reversed-phase high performance liquid chromatography were used to prepare the antioxidant collagen peptide, and an amino acid sequence analyzer was used to determine its amino acid sequence. Mass spectrometry (esi-ms) was used to determine its molecular weight, and the antioxidant capacity of collagen peptides was evaluated using hydroxyl radical, dpph radical, abts radical and superoxide anion radical scavenging experiments and lipid peroxidation inhibition experiments. The results showed that a decapeptide (tfch-p2) was obtained by two-step enzymatic hydrolysis, separation and purification of pepsin and trypsin from tuna bone collagen. Its amino acid sequence was determined to be gly- through amino acid sequence analysis and mass spectrometry (esi-ms). pro-ala-gly-pro-ala-gly-glu-gln-gly (gpagpagqeg), molecular weight is 839.87u ([m+h]+840.68u). The results of in vitro antioxidant experiments show that gpagpagqeg has a strong response to hydroxyl free radicals (ec500.18mg/ml), dpph free radicals (ec500.97mg/ml), abts free radicals (ec500.52mg/ml) and superoxide anion free radicals (ec500. 38mg/ml) has good scavenging effect; gpagpagqeg also shows good lipid peroxidation inhibitory effect. Research shows that the collagen peptide gpagpagqeg has good antioxidant activity and can be used in antioxidant-related functional foods, drugs or food additives.

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Original research was done by Tan Hongliang, Yu Di, Wang Bin, Hu Fayuan, Ma Jianyin

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Cirrhosis, Hepatic Stellate Cells, Collagen, Fuzheng Huayu Prescription, Huangqi Decoction

Abstract

Objective: To observe the therapeutic effect of Fuzheng Huayu prescription combined with Huangqi decoction on rats with experimental cirrhosis, and to explore the characteristics of the therapeutic effect of deficiency-tonifying traditional Chinese medicine on liver cirrhosis. Methods: A rat model of liver cirrhosis was prepared by intraperitoneal injection of dimethylnitrosamine (DMN) for 4 weeks. After the model was formed, the model rats were given Fuzheng Huayu Recipe, Huangqi Decoction, Fuzheng Huayu Recipe and Huangqi Decoction by gavage for 2 weeks. Observe the body weight, liver weight, and spleen weight of rats; HE staining to observe inflammation and pathology of liver tissue; Sirius red staining to observe collagen deposition in liver tissue; The kit detects rat serum liver function (ALT, Alb, T.Bil) and liver tissue SOD activity. and MDA content; Hyp content in liver tissue was determined by hydrochloric acid hydrolysis method; ¦Á-SMA expression in liver tissue was observed by immunohistochemical staining; ¦Á-SMA protein expression in liver tissue was detected by Westernblot. Results: Compared with normal rats, the serum ALT and T.Bil of the model rats were significantly increased, and the Alb content was significantly decreased; the SOD activity of the liver tissue decreased, and the MDA and Hyp contents were significantly increased; the inflammatory lesions and collagen deposition in the liver tissue were obvious. ¦Á-SMA expression was significantly increased. Huangqi decoction, Fuzheng Huayu prescription and their combination can significantly improve the serum liver function of model rats, improve liver lipid peroxidation, reduce liver tissue Hyp content, reduce liver tissue inflammation and collagen deposition, and inhibit ¦Á-SMA expression. In terms of improving liver collagen deposition and inhibiting ¦Á-SMA expression, the combination of the two is better than either alone. Conclusion: Fuzheng Huayu Recipe, Huangqi Decoction, Fuzheng Huayu Recipe and Huangqi Decoction can all improve liver fibrosis in DMN model rats. The combination of the two prescriptions inhibits collagen deposition more significantly than each single prescription group. Part of the mechanism is that the combination of the two prescriptions can improve liver fibrosis. Good at inhibiting hepatic stellate cell activation.

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Original research was done by Wang Luobing, Yan Xiuchuan, Zeng Zhen, Lu Jing, Liu Ping, Liu Chenghai

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Psoralen, Alp, Type I Collagen, Osteocalcin, Gene Expression

Abstract

Objective: To study the effect of psoralen extract on the differentiation of neonatal mouse calvarial MC3T3-E1 cells cultured in vitro. Methods: The mouse osteoblast cell line MC3T3-E1 was used as a cell model for drug screening. The cultured MC3T3-E1 mouse osteoblasts were divided into 4 groups: blank group, psoralen extract (containing crude drug) 0.02, 0.2 , 2g¡¤L-1 different dosage groups. The contents of cell ALP and type I collagen were detected, and real-time PCR was used to determine the effect of psoralen extract on the expression of ALP, type I collagen and osteocalcin mRNA in MC3T3-E1 cells. Results: Psoralen extract crude drug dose 0.2g¡¤L-1 can increase the activity of ALP in MC3T3-E1 cells and promote the secretion of type ¢ñ collagen. At the same time, it significantly promoted the expression of ALP in MC3T3-E1 cells from 7 to 14 days, and the mRNA expression of type I collagen and osteocalcin from 14 to 21 days. Conclusion: The traditional Chinese medicine Psoralen extract can effectively promote the differentiation of osteoblasts and provide a reference for studying the mechanism of action of the traditional Chinese medicine Psoralen.

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Original research was done by Song Qin 1,2, Dong Xiaoping 2, Yu Xiaobing 1,3*

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Astragalus, Injection Sciatic Nerve Injury, Collagen Fibers, Muscle Atrophy

Abstract

Objective To explore the mechanism of astragalus improving muscle atrophy in rats with injection sciatic nerve injury. Methods 24 SD rats were randomly divided into 4 groups: normal group, model group, negative control group and astragalus intervention group. The muscle weight was measured using a balance, the cross-sectional area of muscle fibers was measured with a Leica image analyzer after HE staining, and the changes in collagen fibers in the peroneus longus muscle were observed with VG staining. Results Two weeks after penicillin injection into the sciatic nerve, compared with the normal group, the rats in the model group limped, the peroneus longus muscle mass shrank, the color became darker, the muscle weight decreased, the muscle fiber cross-sectional area decreased, and the collagen fibers proliferated significantly; the negative control group The symptoms were further aggravated; the above changes were significantly improved in the astragalus intervention group. Conclusion Astragalus can improve muscle atrophy caused by sciatic nerve injury caused by penicillin injection by reducing collagen fiber proliferation in rat peroneus longus muscle.

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Original research was done by Yang Shengbo, Mu Jingxia

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

Abstract Using Chinese aloe vera as the test material, a comparative study was conducted on the sensory qualities such as viscosity, color and main nutrients and the main nutrients of the gel raw juice obtained by hot pressing and enzymatic juice making processes after high-temperature sterilization and storage at room temperature in the dark. , changes in functional components. The results show that during the 30-day storage period, the hot-pressed original juice has less nutrient loss than the enzymatically hydrolyzed original juice, has a higher polysaccharide preservation rate, slower decrease in viscosity, and is more stable in color and body. The hot-pressed treatment is used , the original aloe vera gel juice produced by the 90¡ãC, 60 s sterilization process can be stored at room temperature for 20 days with basically unchanged quality.

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About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Molecular Weight Gel Chromatography Of Chrome Leather Residue

Abstract

Using distilled water containing 0.02% sodium azide as the mobile phase and broad distribution T10 and T70 dextran as standard samples, the molecular weight of collagen extracted from chrome leather residue under different conditions was determined by aqueous phase gel chromatography (WPGC). and molecular weight distribution. Within the reaction time and pH value range of the medium set in the experiment, the number average molecular weight (Mn) of collagen is lower than 4000, and the molecular weight distribution is wide (d=Mw/Mn=3.19-8.35)

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Original research was done by Zhang Chunping, Gan Youxin, Gan Chunji, Jiang Tingda

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Inclusion Bodies, Type Iv Collagenase, Fusion Protein, Purification, Renaturation

Abstract

Optimize the preparation process of the anti-type IV collagenase single-chain antibody and lidamycin prosthetic protein fusion protein fv-ldp expressed in the form of inclusion bodies. Optimize its inclusion body preparation and dissolution, immobilized metal chelation chromatography and step-by-step dialysis renaturation process through single factor or orthogonal design, finely purify the protein through sephadexg-75 chromatography, and detect its inclusion body based on saturation binding experiment using flow cytometry. Antigen binding activity. After optimization, the purity of fv-ldp inclusion bodies was 63.9% and the solubility was 95.7%; after purification, the purity of fv-ldp reached more than 95%, the monomer content after renaturation accounted for 60%, and after fine purification, it increased to 85%, which was higher than the initial recombination. The sexual condition is increased by 5.6 times, and it can bind to human lung adenocarcinoma PAA cells and liver cancer bel-7402 cells. The preparation process of fv-ldp has been successfully optimized, laying an experimental foundation for its production and research and development, and also providing a more practical method for the preparation of other miniaturized antibody drugs based on single-chain antibodies expressed in the form of inclusion bodies.

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Original research was done by Gao Ruijuan, Zhao Chunyan, Li Diandong, Zhen Yongsu

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Umbilical Cord, Mesenchymal Stem Cells, Collagenases, Passage Effect, Wharton’S Glue

Abstract

: Objective To observe the effects of different collagenase digestion methods on the isolation and culture results of human umbilical cord Wharton’s jelly mesenchymal stem cells (mesenchymalstem cells, msc), and to identify their differentiation potential; to explore the impact of passage effects on their immune phenotype. Methods: Add type I, type II and type IV collagenase to the prepared umbilical cord specimens, continue digestion for 4 to 18 hours, filter through a sieve, collect cells by centrifugation, resuspend the cells in DMEM/F12 culture medium, and adjust the cell density to 4.8¡Á103 ~1¡Á104/cm2, inoculate and culture, and compare the effects of different digestion methods on isolating Wharton¡¯s Jelly MSC from human umbilical cord. Vonkossa calcium nodule staining and tetracycline fluorescent labeling were used to identify the ability of human umbilical cord Wharton’s Jelly MSCs to differentiate into osteoblasts, and rt-PCR was used to identify their ability to differentiate into cardiomyocytes. Flow cytometry was used to detect the immunophenotypic changes of MSCs after serial passage. Results: Type I collagenase digestion method can obtain a larger number and higher vitality of MSCs from human umbilical cord Wharton’s Jelly, and the time for cells to stretch and the primary culture time are shorter than those of type II collagenase and type IV collagenase digestion. Law. Surface marker analysis showed that as the number of passages increased, the expression percentages of positive markers CD29, CD44, CD73, CD90, and CD105 did not change, while the expression rates of negative markers CD31, CD34, and HLA-DR increased significantly. In vitro induction experiments showed that MSCs derived from human umbilical cord Wharton’s jelly have the ability to differentiate into osteogenic and cardiomyoblast-like cells in vitro. Conclusion Type I collagenase digestion method is simple and easy to perform, causes little damage to cells, and can isolate MSCs from Wharton’s jelly of human umbilical cord stably and efficiently.

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Original research was done by Hong Jingxin, Li Qian, Han Junling

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

Previously (CMJ, 97 (1984), 10717-724) we reported for the first time that after long-term immunization of guinea pigs with human liver membrane specific lipoprotein (Humanliverspecific lipoprotein) and Freund’s complete adjuvant, we observed the process of collagen fiber production by liver cells in vivo. Using the same animal model, we also observed changes in the small bile ducts in the portal area, proving that their epithelial cells can also produce collagen fibers after immune stimulation. Under electron microscopy, the walls of small bile ducts are thickened, and the base of epithelial cells

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Original research was done by Zhao Deyu

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.