Collagen Research

Keywords

Sea Cucumber, Internal Organs, Gelatin Zymogram, Metalloproteinase, Isolation And Purification

Abstract

In view of the autolysis phenomenon of sea cucumbers, the presence of proteases related to collagen degradation in sea cucumber viscera was studied in order to understand the role of endogenous proteases in the autolysis process of sea cucumbers and provide a theoretical basis for elucidating the intrinsic mechanism of autolysis. Through ammonium sulfate salting out, deae-sepharose anion exchange column chromatography, sephacryls-200 gel filtration column chromatography, phenylsepharoseff hydrophobic column chromatography and miniq ion exchange column chromatography, the gelatin-degrading gelatin was isolated and purified from sea cucumber viscera. Protease. The fluorescent substrate was used to study its enzymatic properties and its degradation of sea cucumber body wall collagen. The results show that the optimal temperature of the enzyme is 30¡ãC and the optimal pH is 7.5. It can be completely inhibited by metalloproteinase inhibitors edta and egta, and can also be completely inhibited by serine protease inhibitors such as pmsf and pefablocsc. The substrate specificity showed that the enzyme only decomposed the fluorescent substrate of metalloproteinase, and it was speculated that it was a metalloprotease. The enzyme can effectively decompose sea cucumber body wall collagen at both 4 and 37¡ãC, indicating that it plays an important role in the autolysis process of sea cucumbers.

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Original research was done by Ma Jinhua, Weng Ling, Yan Longjie, Zhang Lingjing, Liu Guangming, Cao Minjie

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Nano-Hydroxyapatite, Alendronate Sodium, Collagen Type I, Osteoblast, Nano-Hydroxyapatite, Alendronate Saium, Collagen Type I, Osteoblast

Abstract

Abstract Purpose: To explore the effect of nano-hydroxyapatite (nHA) coating modified by chemical combination of alendronate sodium (ALN) and type I collagen (COL I) on osteogenesis Effects on cell adhesion, growth and proliferation on their surfaces. Methods: nHA coating, nHA/ALN, nHA/COL I, nHA/ALN/COL I and nHA/COL I/ALN composite membranes were established, osteoblasts were seeded on the surface of the composite membranes, cultured for 7 d and 14 d respectively, and scanned The changes in the adhesion morphology of osteoblasts on the surface of different membrane layers were observed under an electron microscope, and the number of cells on the surface of each membrane layer was quantitatively detected by WST-1 method. Results: The proliferation activity of osteoblasts was the highest on the surface of nHA/ALN/COL I and nHA/COL I/ALN composite membranes (P<0.05). Scanning electron microscopy observed that osteoblasts had the highest proliferation activity on the surfaces of nHA/ALN/COL I and nHA/COL I. The growth status of the surface of the /ALN composite membrane was better than that of other membrane layers. Under the direct action of the COL I membrane, the osteoblasts stretched more completely, and had more pseudopod protrusions and microvilli structures showing a differentiated phenotype. Conclusion: Modification of ALN and COL I on the surface of nHA can synergistically promote the adhesion and proliferation of osteoblasts. nHA/ALN/COL I composite membrane promotes osteoblast adhesion and differentiation For further information of this article and research, feel free to contact our team for asssitance. Original research was done by JIANG Yan, YAO Jiang-Wu  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Apolipoprotein E Gene-Deficient Mice, Cardiac Muscle, Collagen, Cholesterol

Abstract

Abstract: Objective To dynamically observe the changes in cardiac morphology and myocardial collagen in ApoE gene-deficient mice of different ages fed with high cholesterol. Methods ApoE gene-deficient mice (n=6/group) aged 24, 32 and 40 weeks were used as the research subjects, and C57BL/6J mice of the same age were set as controls. Serum cholesterol levels were routinely detected; HE staining was used to observe the morphological changes of the hearts of mice in each group, Masson staining was used to observe myocardial collagen changes, and the KS400 image analysis system was used to measure the myocardial collagen volume fraction (CVF) and the ratio of the collagen area and lumen area around myocardial blood vessels ( PVCA), picric acid Sirius red staining for left ventricular collagen specific staining and quantitative analysis to observe changes in type I and type III collagen. Results The left ventricular anterior wall, left ventricular lateral wall, and left ventricular posterior wall of 40-week-old ApoE-/- mice were thicker than those of 24-week-old ApoE-/- mice (P<0.05); 24-, 32-, and 40-week-old ApoE The collagen content in the myocardium of -/- mice increased with age (P<0.05), and type I collagen gradually increased. Conclusion ApoE gene-deficient mice fed with high cholesterol have an enlarged heart and a significant increase in myocardial collagen content as cholesterol levels increase. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Qin Yanwen; Ye Ping, Wang Luya; Yang Ya  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

[Scorch Resistance, Carboxylic Acid, Acrylic Rubber, Configuration, Scorch Resistance, Carboxylic Acid, Acrylic Rubber, Configuration]

Abstract

A raw material formulation of carboxylic acid acrylate rubber with excellent scorch resistance

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Original research was done by Zhao Min

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Epimedium, Icariin, Type I Procollagen Amino Terminal Peptide, Type I Collagen Cross-Linked Amino Terminal Peptide

Abstract

Objective To study the anti-osteoporosis mechanism of icariin through the changes of type I procollagen amino terminal peptide (PINP) and type I collagen cross-linked amino terminal peptide (NTx) indicators. Methods Ovariectomy was used to induce osteoporosis model in rats, and different extracts of Epimedium were administered to the rats. Changes in PINP, NTx and other related indicators were detected to evaluate the anti-osteoporosis efficacy of Epimedium. Results Compared with the blank control group, the contents of estradiol (E2) and PINP in the model group were significantly reduced (P<0.01), and the level of NTx was increased (P<0.01); compared with the model group, the levels of icariin were low, medium, and The high-dose group could increase E2 and PINP levels (P<0.05) and reduce NTx content (P<0.05) in castrated rats. Conclusion Icariin has estrogen-like effects and can improve osteoporosis in ovariectomized rats. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Zongquan, Song Min, Jiang Yiwei, Lei Bin  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Dunhuang Xiaobi Dingtong Tincture, Collagen-Induced Arthritis, Nitric Oxide, 3′, 5′-Cyclic Adenosine Monophosphate, Prostaglandin E2

Abstract

Objective: To study the mechanism of action of Dunhuang Xiaobi Dingtong Tincture in the treatment of rheumatoid arthritis, and to explore its effects on synovial fluid nitric oxide (NO) and 3′,5′-cyclic glands in collagen-induced arthritis (CIA) rat model. The influence of glycoside acid (cAMP) and prostaglandin E2 (PGE2) content. Methods: A CIA rat model was established, and the double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method was used to detect the cAMP and PGE2 contents, and the nitrite-colorimetric (Griess) method was used to detect the NO content. Results: Dunhuang Xiaobi Dingtong Tincture can significantly reduce the contents of NO and PGE2 in the synovial fluid of CIA rats, and increase the content of cAMP. Conclusion: It is suggested that Dunhuang Xiaobi Dingtong Tincture has a certain inhibitory effect on arthritis in CIA rats. It can reduce the inflammation of joint synovium by inhibiting the contents of NO and PGE2 in rat synovial fluid and increasing the content of cAMP. This may Is one of its mechanisms of action in treating rheumatoid arthritis

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Original research was done by Song Pengcheng, Zeng Zhaoyang, Li Yuwei, Yang Guodong, Mi Zhongxiang, Guo Yingqiang

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Dental Pulp, Tissue Scaffold, Collagen, Bioactive Glass, Cell Proliferation

Abstract

Purpose: To observe the proliferation and differentiation of human dental pulp cells (HDPCs) on bioactive scaffolds. Methods: HDPCs were cultured using enzyme digestion method and passed to the 4th generation for experiments. Immunohistochemistry was used to identify cells and determine the expression rate of stromal precursor cell antigen-1 (stromal precursorantigen-1, stro-1). Three types of scaffolds were used in the experiment, including collagen (collagen, col) scaffold, collagen-bioactive glass (collagen-bioglass, col-bg) scaffold and collagen-bioglass-polycaprolactone (collagen-bioglass-polycaprolactone, col- bg-pcl) stent. HDPCs were implanted into the scaffold, and HDPCs proliferation was measured using the MTT method at 6h, 1d, 3d, 5d, 7d, 14d and 21d, and alkaline phosphatase staining was performed on the 14th day. Results: The HDPCs used in the experiment contained stro-1 positive cells; the proliferation of HDPCs on col-bg scaffolds and col-bg-pcl scaffolds was significantly higher than that on col scaffolds (p<0.05), especially on 14d and 21d col-bg scaffolds and col -The optical density value of the bg-pcl scaffold is 5 times that of the col scaffold; the alkaline phosphatase staining area on the col-bg scaffold and col-bg-pcl scaffold is significantly wider than that of the col scaffold. Conclusion: HDPCs proliferate and differentiate actively on col-bg scaffolds and col-bg-pcl scaffolds, which are better than traditional col scaffolds. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Wei Qiangwei, Dong Yanmei, Chen Xiaofeng, Li Yuli, Miao Guohou  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Muscle, Chinese Mitten Crab, Tenderness, Collagen, Muscle Fiber

Abstract

Abstract: Using three evaluation methods: myofibril fragmentation index (mfi), collagen (total collagen, hot-soluble collagen, thermal residual collagen) and muscle fiber diameter, the tissue of Chinese mitten crab muscles in 4 regions were measured. characteristics, and preliminarily discussed the use of comprehensive index method to compare meat tenderness. The results show that the muscle fiber fragmentation index of Chinese mitten crab muscle is 50-90; the muscle fiber diameter is 350-560 ¦Ìm; the total collagen content is 0.1-0.18g? (100g)-1; The content of hot-soluble collagen is 0.03~0.12g? (100g)-1; The thermal residual collagen content is 0.04~0.1g? (100g)-1. Compared with beef, Chinese mitten crab muscles have thicker muscle fibers and lower collagen content. All three evaluation methods can classify the muscle tenderness levels among river crabs in different regions, but the tenderness rankings among each index are different. Muscle fiber diameter has the highest resolution, while collagen has the lowest resolution. The comprehensive index method is used to evaluate the meat tenderness in descending order: b>c>a>d (male); a>c>b>d (female).

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Original research was done by Zheng Haibo, Xia Wenshui

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Highly Purified, Acellular Porcine Dermal Matrix, Collagen

Abstract

Using traceable pig skin as raw material, the pig skin is highly purified using special chemical, physical and biochemical methods. By observing the thickness and area of the skin piece in the experiment, the content of collagen and non-collagen protein in the waste liquid, and the histological shape Based on the changes in appearance, it was concluded that the best solution for alkali expansion of acellular pig dermal matrix is 4% hydrogen peroxide-6% sodium hydroxide. We also prepared a high-purity acellular porcine dermal matrix and tested its tensile strength of 7.2Mpa, good hygroscopicity, no heavy metals, no oral acute toxicity, clean cell removal and level 1 cytotoxicity, which provides a basis for the future application of acellular porcine dermal matrix. Laying the foundation for applications in the field of biomedical materials

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Original research was done by But Nianhua

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

Recent literature reports that hepatocytes cultured in vitro can produce collagen fibers, but whether hepatocytes in vivo can produce collagen fibers in situ remains unclear. This article reports using light and electron microscopy to observe the in situ production of collagen fibers by liver cells in guinea pig liver damage caused by immune response.

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Original research was done by Zhao Deyu

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.